The drugs ibuprofen and diclofenac were assessed in vivo on adult females of the estuarine crab Neohelice granulata. In a first, preliminary assay comprising 60-d, a significant (p < 0.05) lower content of total vitellogenic proteins was detected in the ovary at 10 mg/L of each drug. In a second 90-d assay, comprising the exposure of crabs to 5 mg/L of each drug during the entire pre-reproductive period, a significant (p < 0.05) decrease in the proportion of vitellogenic oocytes was observed by effect of diclofenac. The same effect was also observed in a third assay only comprising the last month of the pre-reproductive period, at 5 mg/L of diclofenac, and also at a mixture of both drugs; besides, this mixture significantly (p < 0.05) increased the proportion of reabsorbed vitellogenic oocytes. The obtained results indicate that the effect of diclofenac is critical at the final stage of ovarian maturation, when the participation of prostaglandins is relevant.

药物布洛芬和双氯芬酸在河口蟹Neohelice granulata的成年雌性体内进行了评估. 在第一个包含 60 天的初步测定中，在每种药物 10 mg/L 时，卵巢中总卵黄蛋白的含量显着降低（p < 0.05）。在第二个 90 天测定中，包括在整个生殖前期期间将螃蟹暴露于 5 mg/L 的每种药物中，观察到双氯芬酸的作用显着 (p < 0.05) 减少了卵黄卵母细胞的比例。在仅包括生殖前期最后一个月的第三次测定中也观察到相同的效果，在 5 mg/L 双氯芬酸下，以及在两种药物的混合物下；此外，这种混合物显着（p < 0.05）增加了重吸收的卵黄原卵母细胞的比例。所得结果表明，双氯芬酸的作用在卵巢成熟的最后阶段至关重要，