To investigate the universality of mycelium pellet (MP) assisted flocculent activated sludge (FAS) granulation, four different filamentous microbes (Aspergillus niger, Aspergillus fumigatus, Phanerochaete Chrysosporium and Streptomyces albicans) were used for bio-flocculation assisted activated sludge granulation, and the physicochemical properties and biological activity of aerobic granular sludge (AGS) with MP as skeleton were monitored. The results showed that inoculation of MP for an assisted flocculation strategy was better than filamentous spores. Spherical and rod-shaped microbes of FAS alternately adhered to different areas of the MP to form granular sludge with dense structure. Under the optimized conditions, the FAS biomass aggregated on four MPs reached 1.24–1.89 g/g, and the respiratory activity of corresponding MP-based AGS (MP-AGS) were 36.52–56.95 mgO₂/gVSS of specific oxygen consumption rate. Compared to FAS, MP-AGS present better sedimentation performance (45–55 m/h of settling rate) and higher biological enzyme activity related to organic matter degradation and nitrogen conversion (49.82–60.37 mgTF/(gVSS∙h) of dehydrogenase, 0.26–0.42 mgNO₂⁻-N/(min∙mg protein) of nitrate reductase). TN and TP removal efficiencies of MP-AGS were 9.96 %–19.59 % higher than those of FAS under high influent loads. This study confirmed the feasibility of mycelium pellet assisted flocculent activated sludge granulation using different microbes.

研究菌丝体颗粒 (MP) 辅助絮凝活性污泥 (FAS) 造粒的普遍性，四种不同的丝状微生物（黑曲霉、烟曲霉、金黄原毛菌和白色链霉菌）) 用于生物絮凝辅助活性污泥造粒，监测以 MP 为骨架的好氧颗粒污泥 (AGS) 的理化性质和生物活性。结果表明，用于辅助絮凝策略的 MP 接种优于丝状孢子。FAS的球形和棒状微生物交替附着在MP的不同区域，形成结构致密的颗粒污泥。在优化条件下，四种 MPs 上的 FAS 生物量聚集达到 1.24-1.89 g/g，相应的 MP-based AGS (MP-AGS) 的呼吸活性为 36.52-56.95 mgO ₂/gVSS 比耗氧率。与 FAS 相比，MP-AGS 具有更好的沉降性能（45–55 m/h 的沉降速率）和更高的与有机物降解和氮转化相关的生物酶活性（49.82–60.37 mgTF/(gVSS∙h) 的脱氢酶，0.26 –0.42 mgNO ₂ ^- -N/(min∙mg 蛋白质) 的硝酸还原酶)。在高进水负荷下，MP-AGS 的 TN 和 TP 去除效率比 FAS 高 9.96 %–19.59 %。该研究证实了使用不同微生物进行菌丝体颗粒辅助絮凝活性污泥造粒的可行性。