ABSTRACT

Objectives: Mycobacterium paragordonae (MPG) is an emerging and less common type of Non-tuberculous mycobacteria (NTM) and we know little about its characteristics and prognosis, hence we constructed this retrospective cohort study. Methods: MPG was identified using MALD-TOF MS, multi-target combined gene sequencing and WGS. Clinical information was collected, antimicrobial susceptibility testing was measured using the SLOMYCO panel, and optimal growth temperature testing was measured using Lowenstein-Jensen medium. Results: Eight MPGs were isolated from 1730 NTMs (0.46%); the mean age of MPG pulmonary disease (MPG-PD) patients was 42.38 ± 9.92 years, 37.5% were male, and the average BMI was 18.4 ± 0.51 kg/m². All patients had the symptoms of cough and sputum and CT images mainly presented in patchy or streaky shadows, MPG grew at 25°C, 30°C and 37°C, and the optimal growth temperature is 37°C. MPGs were sensitive to clarithromycin, rifabutin, amikacin, linezolid, moxifloxacin, cotrimoxazole and ciprofloxacin, two isolates were resistant to rifampicin. Two patients had follow up information, their imaging remained stable during the follow-up. Conclusions: MPG-PD is a rare NTM disease and is more likely to develop in middle-aged, female, and low BMI patients. The patients present with no specific features within the symptoms as well as the CT imaging. The optimal growth temperature of MPG is at 37°C, MPG-PD has excellent sensitivity to drugs recommended by CLSI and presents with a stable disease.

摘要

目的：副戈登分枝杆菌(MPG) 是一种新兴且不太常见的非结核分枝杆菌 (NTM)，我们对其特征和预后知之甚少，因此我们构建了这项回顾性队列研究。方法：使用 MALD-TOF MS、多靶点联合基因测序和 WGS 鉴定 MPG。收集临床信息，使用 SLOMYCO 面板测量抗菌药物敏感性测试，使用 Lowenstein-Jensen 培养基测量最佳生长温度测试。结果：从 1730 个 NTM 中分离出 8 个 MPG（0.46%）；MPG肺病（MPG-PD）患者的平均年龄为42.38±9.92岁，37.5%为男性，平均BMI为18.4±0.51 kg/m ². 所有患者均有咳嗽咳痰症状，CT图像主要表现为斑片状或条纹状阴影，MPG在25°C、30°C和37°C生长，最佳生长温度为37°C。MPGs对克拉霉素、利福布丁、阿米卡星、利奈唑胺、莫西沙星、复方新诺明和环丙沙星敏感，2株对利福平耐药。2例患者有随访信息，随访期间影像保持稳定。结论：MPG-PD 是一种罕见的 NTM 疾病，更容易发生在中年、女性和低 BMI 患者中。患者在症状和 CT 成像中没有特定的特征。MPG的最佳生长温度为37°C，MPG-PD对CLSI推荐的药物具有极好的敏感性，并呈现稳定的疾病状态。