Aging and age-related diseases have been linked to microbial dysbiosis with changes in blood bacterial DNA concentration. This condition may promote chronic low-grade inflammation, which can be further aggravated by antioxidant nutrient deficiency. Low plasma carotenoids are associated with an increased risk of inflammation and cellular damage and predict mortality. However, no evidence is yet available on the relationship between antioxidants and the blood bacterial DNA (BB-DNA). Therefore, this study aimed to compare BB-DNA from (i) GO (nonagenarian offspring), (ii) age matched controls [(Randomly recruited Age-Stratified Individuals from the General population (RASIG)] and (iii) Spouses of GO (SGO) recruited in the MARK-AGE project, as well as to investigate the association between BB-DNA, behavior habits, Charlson comorbidity index (CCI), leucocyte subsets, and the circulating levels of some antioxidants and oxidative stress markers. BB-DNA was higher in RASIG than GO and SGO, while GO and SGO participants showed similar values. BB-DNA increased in smokers and in males with CCI≥2 compared to those with CCI≤1 within RASIG. Moreover, BB-DNA was positively associated with lymphocyte, neutrophil and monocyte counts, but not with self-reported dietary habits. Higher quartiles of BB-DNA were associated with low lutein and zeaxanthin and elevated malondialdehyde plasma concentrations in RASIG. BB-DNA was also positively correlated with nitric oxide levels. Herein, we provide evidence of a reduced BB-DNA in individuals from long-living families and their spouses, suggesting a decreased microbial dysbiosis and bacterial systemic translocation. BB-DNA was also associated with smoking, CCI, leukocyte subsets and some redox biomarkers in older subjects.

中文翻译：

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老年受试者和九十多岁后代的细菌 DNA 血症以及与氧化还原生物标志物的关联：MARK-AGE 研究的结果

衰老和与年龄相关的疾病与血液细菌 DNA 浓度变化引起的微生物失调有关。这种情况可能会促进慢性低度炎症，而抗氧化营养素缺乏会进一步加剧这种炎症。低血浆类胡萝卜素与炎症和细胞损伤的风险增加有关，并预测死亡率。然而，目前还没有关于抗氧化剂和血液细菌 DNA (BB-DNA) 之间关系的证据。因此，本研究旨在比较来自 (i) GO（九十多岁的后代），(ii) 年龄匹配对照 [（从普通人群中随机招募的年龄分层个体 (RASIG)] 和 (iii) GO 的配偶的 BB-DNA（ SGO) 在 MARK-AGE 项目中招募，以及调查 BB-DNA、行为习惯、Charlson 合并症指数 (CCI) 之间的关联，白细胞亚群，以及一些抗氧化剂和氧化应激标志物的循环水平。RASIG 中的 BB-DNA 高于 GO 和 SGO，而 GO 和 SGO 参与者表现出相似的值。在 RASIG 中，与 CCI ≤ 1 的人群相比，吸烟者和 CCI ≥ 2 的男性的 BB-DNA 增加。此外，BB-DNA 与淋巴细胞、中性粒细胞和单核细胞计数呈正相关，但与自我报告的饮食习惯无关。较高四分位数的 BB-DNA 与 RASIG 中的低叶黄素和玉米黄质以及升高的丙二醛血浆浓度相关。BB-DNA 也与一氧化氮水平呈正相关。在此，我们提供证据表明长寿家庭及其配偶的个体 BB-DNA 减少，表明微生物失调和细菌系统易位减少。BB-DNA 也与吸烟、CCI、