The experiments were conducted at Research Farm, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India. Infectivity and effectiveness of entomopathogenic nematode (EPN), Steinernema asiaticum infective juveniles (IJs) were evaluated against the diamondback moth (DBM), Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) as foliar spray on cabbage plants in screen house with single (30,000 IJs per plant), two (15,000 + 15,000 IJs per plant) or three (10,000 + 10,000 + 10,000 IJs per plant) applications. In the field trial, S. asiaticum (25,000 or 50,000 IJs per plant) and Malathion 50 EC (0.05%) singly or in combination at half the concentrations were used. In screen house trial, all the treatments resulted in significantly high larval mortality than untreated plants. Split concentration application (15,000 + 15,000 IJs per plant) gave better DBM larval mortality (48.33%) compared to single application (36.00% at 30,000 IJs per plant). Comparing the two and three split applications, 15,000 IJs was significantly better (23.33% mortality) than 10,000 IJs (13.33%). In the field trial, S. asiaticum alone at 50,000 IJs resulted in 28.8% insect mortality compared to 18.0% in Malathion at 0.05%. Best results (37.5% mortality) were obtained by using nematode IJs at 25,000 per plant in combination with half the recommended concentration of Malathion (0.025%) leading to synergistic effect. The study revealed that split application (15,000 + 15,000 IJs) of EPN S. asiaticum IJs proved better than single application of IJs at the same concentration (30,000 IJs) in the management of P. xylostella larvae in the screen house conditions. In the field experiment, the best effectiveness was obtained in combination of half the concentrations of EPN and insecticide (S. asiaticum IJs @ 25,000 per plant + Malathion @ 0.025%).

中文翻译：

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昆虫病原线虫 Steinernema asiaticum 对小菜蛾 Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) 在筛房和田间条件下的评价

实验在印度希萨尔的乔杜里查兰辛格哈里亚纳农业大学研究农场进行。对小菜蛾 (DBM)、小菜蛾 (Linnaeus) (鳞翅目: Plutellidae) 对小菜蛾 (DBM)、小菜蛾 (Linnaeus) (鳞翅目: Plutellidae) 的昆虫病原线虫 (EPN)、Steinernema asiaticum infective juveniles (IJs) 的感染性和有效性进行了评估，作为叶面喷雾剂对单株 (30,000) 的卷心菜植物进行了评估每个工厂的 IJs）、两个（每个工厂的 15,000 + 15,000 个 IJs）或三个（每个工厂的 10,000 + 10,000 + 10,000 个 IJs）应用程序。在田间试验中，使用 S. asiaticum（每株植物 25,000 或 50,000 IJs）和马拉硫磷 50 EC（0.05%）单独或以一半的浓度组合使用。在筛选房试验中，所有处理导致幼虫死亡率显着高于未经处理的植物。分离浓度应用（15,000 + 15，每株植物 000 IJs）与单次施用（每株植物 30,000 IJs 时为 36.00%）相比，DBM 幼虫死亡率更高（48.33%）。比较两次和三次拆分申请，15,000 名 IJ（死亡率为 23.33%）明显优于 10,000 名（13.33%）。在田间试验中，50,000 IJs 的 S. asiaticum 导致 28.8% 的昆虫死亡率，而马拉硫磷为 18.0%，为 0.05%。通过使用每株植物 25,000 条线虫 IJ 和一半推荐浓度的马拉硫磷 (0.025%) 获得最佳结果（37.5% 死亡率），从而产生协同效应。研究表明，在筛房条件下对小菜蛾幼虫的管理中，EPN S. asiaticum IJs 的分开施用（15,000 + 15,000 IJs）证明比在相同浓度（30,000 IJs）下单独施用 IJs 更好。在田间实验中，