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Quaterization Derivatization with Bis(Pyridine) Iodine Tetrafluoroboride: High-Sensitivity Mass Spectrometric Analysis of Unsaturated Fatty Acids in Human Thyroid Tissues
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-02 , DOI: 10.1021/acs.analchem.2c01519 Jie Cheng 1 , Yuling Li 1 , Yunjun Wang 2 , Jing Zhang 1 , Tuanqi Sun 2 , Li Zhang 1 , Yinlong Guo 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-08-02 , DOI: 10.1021/acs.analchem.2c01519 Jie Cheng 1 , Yuling Li 1 , Yunjun Wang 2 , Jing Zhang 1 , Tuanqi Sun 2 , Li Zhang 1 , Yinlong Guo 1
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Accurate quantification of disease-related unsaturated fatty acids (UFAs) in biomedical samples plays an important role in clinical diagnosis. Here, we reported a quaterization derivatization-stable isotope labeling strategy for accurate quantitative analysis of UFAs by high-performance liquid chromatography-mass spectrometry. [d0]/[d10]-Bis(pyridine) iodine tetrafluoroboride ([d0]/[d10]-IPy2BF4) was employed as the carbon–carbon double bond derivatization reagent with high efficiency and high specificity, to introduce a charge tag on UFAs and avoid the interference of saturated fatty acids. After labeling, the detection sensitivity was significantly enhanced by up to three orders of magnitude compared to intact UFAs. The standard curves showed good linearity (R2 > 0.999) over a wide concentration range. This strategy was successfully applied to determine the content of 12 UFAs in human thyroid carcinoma and para-carcinoma tissues. A significant difference was found in the content of several UFAs between these two kinds of tissues (p < 0.05). These results indicated that the proposed strategy may be valuable for the discovery of abnormal UFA content in early clinical diagnosis.
中文翻译：
双（吡啶）碘四氟硼化物的季化衍生化：人体甲状腺组织中不饱和脂肪酸的高灵敏度质谱分析
准确定量生物医学样品中与疾病相关的不饱和脂肪酸 (UFA) 在临床诊断中起着重要作用。在这里，我们报告了一种四化衍生稳定同位素标记策略，用于通过高效液相色谱-质谱法对 UFA 进行准确定量分析。[ d 0 ]/[ d 10 ]-双(吡啶)碘四氟硼化物([ d 0 ]/[ d 10 ]-IPy 2 BF 4) 被用作高效、高特异性的碳-碳双键衍生试剂，在 UFA 上引入电荷标签，避免饱和脂肪酸的干扰。标记后，与完整的 UFA 相比，检测灵敏度显着提高了三个数量级。标准曲线在较宽的浓度范围内显示出良好的线性 ( R 2 > 0.999)。该策略成功应用于测定人甲状腺癌和癌旁组织中 12 种 UFA 的含量。这两种组织之间的几种UFA含量存在显着差异（p< 0.05)。这些结果表明，所提出的策略对于在早期临床诊断中发现异常 UFA 含量可能是有价值的。
更新日期：2022-08-02
中文翻译：
双（吡啶）碘四氟硼化物的季化衍生化：人体甲状腺组织中不饱和脂肪酸的高灵敏度质谱分析
准确定量生物医学样品中与疾病相关的不饱和脂肪酸 (UFA) 在临床诊断中起着重要作用。在这里，我们报告了一种四化衍生稳定同位素标记策略，用于通过高效液相色谱-质谱法对 UFA 进行准确定量分析。[ d 0 ]/[ d 10 ]-双(吡啶)碘四氟硼化物([ d 0 ]/[ d 10 ]-IPy 2 BF 4) 被用作高效、高特异性的碳-碳双键衍生试剂，在 UFA 上引入电荷标签，避免饱和脂肪酸的干扰。标记后，与完整的 UFA 相比，检测灵敏度显着提高了三个数量级。标准曲线在较宽的浓度范围内显示出良好的线性 ( R 2 > 0.999)。该策略成功应用于测定人甲状腺癌和癌旁组织中 12 种 UFA 的含量。这两种组织之间的几种UFA含量存在显着差异（p< 0.05)。这些结果表明，所提出的策略对于在早期临床诊断中发现异常 UFA 含量可能是有价值的。
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