Gas pressure is a promising signal readout mode in point-of-care testing for its merits such as rapidity, simplicity, affordability, and no need for sophisticated instrumentation. Herein, a gas pressure sensor for multiplexed detection of pathogenic bacteria was developed on a hydrogel platform. Spherical and square hydrogel pellets prepared by cross-linking of sodium alginate were functionalized with nisin and ConA for the capture of Staphylococcus aureus and Escherichia coli O157:H7, respectively. By using the shape-encoded functional hydrogel pellets and aptamer-modified platinum-coated gold nanoparticles (Au@PtNPs), a dual-molecule recognition mode was established for rapid and specific detection of the two pathogenic bacteria. Au@PtNPs were applied as signal probes to efficiently catalyze the decomposition of H₂O₂ for generating abundant O₂, which was converted into an amplified gas pressure signal. In two closed containers, the significant gas pressure signals were monitored with a portable pressure meter to quantitate the two pathogenic bacteria. The sensor was successfully applied to detect the pathogenic bacteria in various environmental, biological, and food samples. Thus, the proof-of-principle work paves a new avenue for multiplexed detection of pathogenic bacteria with shape-encoded hydrogel pellets combined with gas pressure signal readout.

中文翻译：

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形状编码功能性水凝胶颗粒用于使用气压传感器多重检测致病菌

气压是即时检测中一种很有前途的信号读出模式，因为它具有快速、简单、经济实惠且不需要复杂仪器等优点。在此，在水凝胶平台上开发了一种用于病原菌多重检测的气压传感器。通过海藻酸钠交联制备的球形和方形水凝胶颗粒用乳链菌肽和 ConA 进行功能化，用于捕获金黄色葡萄球菌和大肠杆菌O157：H7，分别。通过使用形状编码的功能性水凝胶颗粒和适配体修饰的铂包覆金纳米粒子（Au@PtNPs），建立了一种双分子识别模式，用于快速和特异性检测两种致病菌。Au@PtNPs作为信号探针有效催化H ₂ O ₂分解产生大量O ₂, 它被转换成放大的气体压力信号。在两个密闭容器中，用便携式压力计监测显着的气压信号，以对两种致病菌进行定量。该传感器已成功应用于检测各种环境、生物和食品样品中的致病菌。因此，原理验证工作为使用形状编码水凝胶颗粒结合气压信号读出来多重检测致病菌铺平了道路。