In order to develop ^99mTc-labeled complexes with bisphosphonate isocyanide as novel bone imaging agents, two bisphosphonate isocyanide derivatives (CNALN and CNPAM) were synthesized and radiolabeling was performed for preparing the corresponding [^99mTc]Tc(I) complexes. [^99mTc]Tc-CNALN and [^99mTc]Tc-CNPAM were obtained with high radiochemical purity and showed good in vitro stability. Both of them were hydrophilic and had high affinity to hydroxyapatite. The biodistribution studies in mice revealed [^99mTc]Tc-CNALN showed higher bone/background ratios at 60 min post-injection. In single photon emission computed tomography (SPECT) imaging study, [^99mTc]Tc-CNALN had an obvious accumulation in bone, suggesting it would be a promising bone-seeking agent.

为了开发^99m Tc 标记的双膦酸盐异氰化物复合物作为新型骨显像剂，合成了两种双膦酸盐异氰化物衍生物（CNALN 和 CNPAM），并进行了放射性标记以制备相应的 [ ^99m Tc]Tc(I) 配合物。[ ^99m Tc]Tc-CNALN和[ ^99m Tc]Tc-CNPAM均获得高放射化学纯度，并表现出良好的体外稳定性。它们都是亲水性的并且对羟基磷灰石具有高亲和力。小鼠体内的生物分布研究表明，[ ^99m Tc]Tc-CNALN 在注射后 60 分钟显示出更高的骨/背景比率。在单光子发射计算机断层扫描 (SPECT) 成像研究中，[ ^99mTc]Tc-CNALN在骨中有明显的积累，表明它是一种很有前途的寻骨剂。