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Effects of Relative Humidity and Paper Geometry on the Imbibition Dynamics and Reactions in Lateral Flow Assays
Langmuir ( IF 3.7 ) Pub Date : 2022-08-01 , DOI: 10.1021/acs.langmuir.2c01017 Debayan Das 1 , Tarun Singh 2 , Isteaque Ahmed 3 , Manaswini Masetty 3 , Aashish Priye 3
Langmuir ( IF 3.7 ) Pub Date : 2022-08-01 , DOI: 10.1021/acs.langmuir.2c01017 Debayan Das 1 , Tarun Singh 2 , Isteaque Ahmed 3 , Manaswini Masetty 3 , Aashish Priye 3
Affiliation
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Lateral flow assays and paper microfluidics have the potential to replace benchtop instrumented medical diagnostic systems with instrument-free systems that rely on passive transport of liquid through micro-porous paper substrates. Predicting the imbibition dynamics of liquid through dry paper substrates is mostly modeled through the Lucas–Washburn (LW) equations. However, the LW framework assumes that the fluid front exhibits a sharp boundary between the dry and wet phases across the liquid imbibition interface. Additionally, the relative humidity in the environment results in moisture trapped within the pores of the paper substrates as the paper attains an equilibrium with the ambient air. Here, we apply a two-phase transport framework based on Brooks and Corey’s model to capture imbibition dynamics on partially saturated paper substrates. The model is experimentally validated and is then used to predict the liquid–paper imbibition dynamics in simulated environments with 1–70% relative humidity. The model was also used to determine the saturation gradient of liquid along the imbibition interface of the paper substrate. Insights from these studies enabled us to determine the mechanism of the liquid transport in partially saturated porous paper substrates. The model also enabled us to evaluate the optimal paper shapes and relative humidity of the environment that maximize imbibition rates and minimize imbibition front broadening. Finally, we evaluate the effect of moisture content of paper on the rate of paper-based biochemical reaction by amplifying a sequence of the SARS-CoV-2 RNA target via reverse transcriptase loop-mediated isothermal amplification. Taken together, this study provides some important guidelines to academic and applied researchers working in point-of-care diagnostics to develop paper-based testing platforms that are capable of functioning in a robust manner across multiple environmental conditions.
中文翻译：
相对湿度和纸的几何形状对侧向流动分析中的渗吸动力学和反应的影响
侧向流动分析和纸微流体有可能用无仪器系统取代台式仪器医疗诊断系统，该系统依赖于液体通过微孔纸基材的被动传输。预测液体通过干纸基材的吸入动力学主要通过 Lucas-Washburn (LW) 方程建模。然而，LW 框架假设流体前沿在液体吸入界面的干相和湿相之间表现出尖锐的边界。此外，环境中的相对湿度导致纸张与环境空气达到平衡时，水分被困在纸张基材的孔隙中。在这里，我们应用基于 Brooks 和 Corey 模型的两相传输框架来捕捉部分饱和纸基材上的吸入动力学。该模型经过实验验证，然后用于预测相对湿度为 1-70% 的模拟环境中的液体 - 纸吸入动力学。该模型还用于确定液体沿纸基材的渗吸界面的饱和梯度。这些研究的见解使我们能够确定部分饱和多孔纸基材中液体传输的机制。该模型还使我们能够评估最佳的纸张形状和环境的相对湿度，以最大限度地提高吸水率并最大限度地减少吸水前沿的扩大。最后，我们通过逆转录酶环介导的等温扩增扩增 SARS-CoV-2 RNA 靶标序列，评估纸的水分含量对纸基生化反应速率的影响。综合起来，
更新日期：2022-08-01
中文翻译：
相对湿度和纸的几何形状对侧向流动分析中的渗吸动力学和反应的影响
侧向流动分析和纸微流体有可能用无仪器系统取代台式仪器医疗诊断系统，该系统依赖于液体通过微孔纸基材的被动传输。预测液体通过干纸基材的吸入动力学主要通过 Lucas-Washburn (LW) 方程建模。然而，LW 框架假设流体前沿在液体吸入界面的干相和湿相之间表现出尖锐的边界。此外，环境中的相对湿度导致纸张与环境空气达到平衡时，水分被困在纸张基材的孔隙中。在这里，我们应用基于 Brooks 和 Corey 模型的两相传输框架来捕捉部分饱和纸基材上的吸入动力学。该模型经过实验验证，然后用于预测相对湿度为 1-70% 的模拟环境中的液体 - 纸吸入动力学。该模型还用于确定液体沿纸基材的渗吸界面的饱和梯度。这些研究的见解使我们能够确定部分饱和多孔纸基材中液体传输的机制。该模型还使我们能够评估最佳的纸张形状和环境的相对湿度，以最大限度地提高吸水率并最大限度地减少吸水前沿的扩大。最后，我们通过逆转录酶环介导的等温扩增扩增 SARS-CoV-2 RNA 靶标序列，评估纸的水分含量对纸基生化反应速率的影响。综合起来，
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