A turn-on fluorescent probe with high selectivity for Hg2+ and its applications in living cells,RSC Advances

当前位置： X-MOL 学术 › RSC Adv. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

A turn-on fluorescent probe with high selectivity for Hg2+ and its applications in living cells
RSC Advances ( IF 3.9 ) Pub Date : 2022-08-01 , DOI: 10.1039/d2ra02185d
Bin Li ₁ , Fuli Tian ₂ , Yupeng Hua ₁

Affiliation

A novel fluorescent probe L with a rhodamine B lactam structure modified with 3-methyl-2-thiophenecarboxaldehyde has been prepared based on the thiophilicity of Hg²⁺. The probe L exhibits a unique response with an “off–on-type” mode towards Hg²⁺ among other biologically relevant metal cations. The limit of detection (LOD) for probe L is 1.5 ppb. In addition, in the presence of Hg²⁺, the probe L shows a colorimetric response from colorless to pink. The recognition behavior of probe L towards Hg²⁺ has been investigated by ¹H NMR titration experiments, Job's plot, and MS and IR analyses. As a result, the ligation between the probe and Hg²⁺ leads to the scission of the spirolactam bond of free L and the restoration of its conjugated structure, which can give rise to the fluorescence enhancement of the probe L. Besides, it also can be used as a sensitivity probe in living cells for Hg²⁺ sensing, which can meet various needs in genetic and environmental samples.

中文翻译：

Hg2+高选择性开启荧光探针及其在活细胞中的应用

^{基于Hg 2+}的亲硫性，制备了一种新型荧光探针L，其具有3-甲基-2-噻吩甲醛修饰的罗丹明B内酰胺结构。探针L对 Hg ²⁺在其他生物学相关的金属阳离子中表现出独特的响应，具有“关-开”模式。探针L的检测限 (LOD) 为1.5 ppb。此外，在Hg ²⁺的存在下，探针L显示出从无色到粉红色的比色响应。探针L对 Hg ²⁺的识别行为已被¹H NMR 滴定实验、乔布图以及 MS 和 IR 分析。结果，探针与Hg ²⁺的连接导致游离L的螺内酰胺键断裂，并恢复其共轭结构，从而引起探针L的荧光增强。此外，它还可作为活细胞中Hg ²⁺传感的灵敏度探针，可满足遗传和环境样本的各种需求。

更新日期：2022-08-01

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11