Reduction of environmental pollution sources is the main target of green chemistry; it is applied across the life cycle of a chemical product. Scientists try to switch to eco-friendly practices to diminish the negative impact of chemicals and solvents on the environment. Analytical chemistry is one of the main fields that mounted green chemistry approach. In this work, sensitive and selective green chromatographic methods with UV detection are described for the simultaneous determination of aspirin, rosuvastatin and clopidogrel. The first proposed method is an RP-HPLC one, which was described and successfully validated for the simultaneous separation and determination of the three components on Prontosil Hyperchom C18 (250 × 4.6 mm, 5 μm) column using an isocratic elution. The second method was a TLC spectrodensitometry in which the three components were separated, identified and quantified. The drugs were applied on silica gel plates, and developed using n-heptane: acetone: glacial acetic acid (60:40:0.4, by volume). The three drugs resolved bands were quantified by spectrodensitometric scanning at 240 nm. Both methods were validated according to ICH, statistically compared to the official methods with full greenness investigation to confirm that the proposed methods are viable alternatives for quality assessment of this combination.

中文翻译：

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同时测定三级混合物中阿司匹林、瑞舒伐他汀和氯吡格雷的绿色色谱方法的开发和验证


减少环境污染源是绿色化学的主要目标；它适用于化学产品的整个生命周期。科学家们试图转向环保做法，以减少化学品和溶剂对环境的负面影响。分析化学是应用绿色化学方法的主要领域之一。在这项工作中，描述了采用紫外检测的灵敏、选择性绿色色谱方法，用于同时测定阿司匹林、瑞舒伐他汀和氯吡格雷。第一个提出的方法是 RP-HPLC 方法，该方法已被描述并成功验证，可使用等度洗脱在 Prontosil Hyperchom C18 (250 × 4.6 mm, 5 μm) 色谱柱上同时分离和测定三种组分。第二种方法是薄层色谱光谱密度测定法，其中三种成分被分离、鉴定和定量。将药物涂在硅胶板上，并使用正庚烷：丙酮：冰醋酸（60：40：0.4，体积比）显色。通过 240 nm 处的光谱光密度扫描对三种药物解析的条带进行定量。这两种方法均根据 ICH 进行了验证，并与经过全面绿色度调查的官方方法进行了统计比较，以确认所提出的方法是该组合质量评估的可行替代方案。