Using intracellular-controlled photochemistry to track dynamic organelle processes is gaining attention due to its broad applications. However, most of the employed molecular probes usually require toxic photosensitizers and complex bioanalytical protocols. Here, the synthesis and performance of two new subcellular probes (MitoT1 and MitoT2) are described. The probes undergo photooxidation in the damaged tissue of zebrafish, a model system for tissue regeneration studies. Using high-resolution confocal microscopy and fluorescence spectroscopy, we combine the mentioned photoinduced interconversion at the homeostatic membrane viscosity to track singlet oxygen activity selectively. The continuous and real-time biosensing method reported here provides a new approach for simultaneously detecting endogenous singlet oxygen and viscosity status.

中文翻译：

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线粒体辅助光氧化在稳态膜微粘度下追踪单线态氧

由于其广泛的应用，使用细胞内控制的光化学来跟踪动态细胞器过程正在受到关注。然而，大多数使用的分子探针通常需要有毒的光敏剂和复杂的生物分析方案。在这里，两种新的亚细胞探针（MitoT1和MitoT2 ）的合成和性能) 进行了描述。探针在斑马鱼受损组织中发生光氧化，斑马鱼是组织再生研究的模型系统。使用高分辨率共聚焦显微镜和荧光光谱，我们将提到的光诱导相互转换结合在稳态膜粘度下，以选择性地跟踪单线态氧活性。这里报道的连续实时生物传感方法为同时检测内源性单线态氧和粘度状态提供了一种新方法。