The present study describes the development of a fit-for-purpose analytical procedure for the detection of Ag NPs in different marine organisms by Solid Sampling Continuous Source High Resolution Atomic Absorption Spectrometry (SS-CS-HR-AAS). The detection is based on the observation of the Ag absorption peak and its atomization delay t_ad which is different for ionic Ag and Ag NPs. The temperature program was optimized in order to achieve the maximum difference between the t ad (Δt_ad ). The method was first developed using biota CRMs spiked with different Ag NPs standard solutions or Ag⁺ , at the same concentration. Then, laboratory exposure experiments were performed on mussels and marine sponges. The results showed that the developed methodology is suitable for the detection of Ag NPs for both groups of organisms, showing Δt_ad up to 3.1 s. The developed method is therefore a promising tool to assess the presence of AgNPs in marine invertebrates.

本研究描述了通过固体采样连续源高分辨率原子吸收光谱法 (SS-CS-HR-AAS) 检测不同海洋生物中的 Ag NPs 的适用分析程序的开发。检测基于对 Ag 吸收峰及其原子化延迟 t _ad的观察，这对于离子 Ag 和 Ag NPs 是不同的。对温度程序进行了优化，以实现 t ad (Δt _ad ) 之间的最大差异。该方法最初是使用添加了不同 Ag NPs 标准溶液或 Ag ^{+的 biota CRM 开发的。}，浓度相同。然后，对贻贝和海绵进行了实验室暴露实验。结果表明，所开发的方法适用于检测两组生物的Ag NPs，显示Δt _ad高达3.1 s。因此，所开发的方法是评估海洋无脊椎动物中 AgNPs 存在的有前途的工具。