In the present study AIA25a strain has been isolated from the rhizospheric soil actinomycetes from selected locations of Rajasthan. Antimicrobial activity test of AIA25a against bacterial strains S. aureus (MTCC 3160), P. aeruginosa (MTCC 1688), P. mirabilis (ITCC 425) has been carried out. A good activity is shown by chloroform crude extract against P. aeruginosa (IZ=13.4±0.3mm), moderate activity (IZ=11.1±0.1) is shown against S. aureus and no activity is observed against P. mirabilis strain. Different compounds isolation is executed with the help of chloroform solvent. Using GC-MS identification has been carried out for compounds present in AIA25a strain followed by partial purification of compounds. With the help of HPTLC method partial purification of compounds has been achieved and followed by GC-MS analysis for identification of separated compounds. The Bioautography technique has been applied for the detection of antimicrobial spot of partial purified compounds of crude extract of AIA25a isolate. Results of this study show the presence of various compounds in the rhizospheric soil of Rajasthan.

中文翻译：

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使用 HPTLC 和 GCMS 技术对放线菌 AIA25a 分离物的生物活性化合物进行定性评估

在本研究中，AIA25a 菌株已从拉贾斯坦邦选定地点的根际土壤放线菌中分离出来。已进行 AIA25a 对金黄色葡萄球菌 (MTCC 3160)、铜绿假单胞菌 (MTCC 1688)、奇异假单胞菌 (ITCC 425) 的抗菌活性测试。氯仿粗提物对铜绿假单胞菌表现出良好的活性（IZ=13.4±0.3mm），对金黄色葡萄球菌表现出中等活性（IZ=11.1±0.1），对奇异假单胞菌菌株未观察到活性。在氯仿溶剂的帮助下进行不同化合物的分离。使用 GC-MS 对 AIA25a 菌株中存在的化合物进行了鉴定，然后对化合物进行了部分纯化。在 HPTLC 方法的帮助下，已经实现了化合物的部分纯化，然后通过 GC-MS 分析来鉴定分离的化合物。Bioautography 技术已应用于检测 AIA25a 分离物粗提物中部分纯化化合物的抗菌点。这项研究的结果表明，拉贾斯坦邦的根际土壤中存在各种化合物。