Different administration approaches were investigated for the selection of bupivacaine administration type and a sensitive high-performance liquid chromatographic (HPLC) method has been developed. Developed method was validated and applied for the determination of bupivacaine in rabbit aqueous humor. The separation was achieved using a XTerra, C8 (250 × 8 mm i.d., particle size 5 μm) analytical column with a mobile phase consisted of acetonitrile and sodium dihydrogen phosphate (pH = 3.0, 20 mM; 30:70, v/v). Bupivacaine detection was performed by Diode Array detector (DAD) at 220 nm. The retention times for bupivacaine is 15.886 min. HPLC-DAD method was linear in the range of 75–4000 ng/mL. The limit of detection was 25 ng/mL and the limit of quantification of bupivacaine was found to be 75 ng/mL (relative standard deviation, RSD ≤ 15%, n = 6). In intra-day and inter-day precision and accuracy analysis, the RSD was found to be in the range of 0.96 and 7.98%, the bias values were 0.64 and 3.33%. Method was carried out for three different type of bupivacaine application because of the investigation of effective drug administration. Twenty aqueous humor samples were in the range of 0.642 and 5.124 μg/mL.

中文翻译：

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布比卡因不同给药途径房水的色谱分析

研究了不同的给药方法来选择布比卡因给药类型，并开发了一种灵敏的高效液相色谱 (HPLC) 方法。所建立的方法经过验证并应用于兔房水中布比卡因的测定。使用 XTerra, C8（250 × 8 mm 内径，粒径 5 μm）分析柱和由乙腈和磷酸二氢钠组成的流动相（pH = 3.0, 20 mM; 30:70, v/v）实现分离。布比卡因检测通过二极管阵列检测器 (DAD) 在 220 nm 处进行。布比卡因的保留时间为 15.886 分钟。HPLC-DAD 方法在 75–4000 ng/mL 范围内呈线性。检测限为 25 ng/mL，布比卡因的定量限为 75 ng/mL（相对标准偏差，RSD ≤ 15%，n = 6）。日内和日间精密度和准确度分析中，RSD分别为0.96%和7.98%，偏倚值为0.64%和3.33%。为了研究有效的给药方式，对三种不同类型的布比卡因应用进行了该方法。20 个房水样本的浓度范围为 0.642 至 5.124 μg/mL。