With the frequent detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in dwellings and wastewater, the risk of transmission of environmental contaminants is of great concern. Fast, simple and sensitive sensors are essential for timely detecting infection and controlling transmission through environment fomites. Herein, we developed a Surface Enhanced Raman Scattering (SERS) aptasensor, which can realize ultrasensitive and rapid assay of SARS-CoV-2 viral particles. In this strategy, we designed a novel locking amplifier which is activated only in the presence of virus by aptamer recognition. The reaction process was carried out though one-pot method at 37 °C, which can save time and resources. In addition, magnetic beads used in reaction system can simplify operation, as well as provide ideas for developing biosensing robots via magnetic field. This SERS aptasensor can detect SARS-CoV-2 virus with a LOD of 260 TU/µL within 40 min in the linear range of 625–10,000 TU/µL. Therefore, this convenience, speediness, sensitivity, and selectivity of detection has great prospects in analyzing SARS-CoV-2 viral particles or other viruses in environment as well as monitoring of environmental virus sources.

随着住宅和废水中频繁检测到严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)，环境污染物传播的风险备受关注。快速、简单且灵敏的传感器对于及时检测感染和控制通过环境污染物的传播至关重要。在此，我们开发了一种表面增强拉曼散射（SERS）适体传感器，可以实现对SARS-CoV-2病毒颗粒的超灵敏和快速检测。在这个策略中，我们设计了一种新型锁定放大器，它仅在病毒存在的情况下通过适体识别被激活。反应过程在37℃下通过一锅法进行，可以节省时间和资源。此外，磁珠用于反应系统可以简化操作，也为开发利用磁场的生物传感机器人提供了思路。该SERS适体传感器可以在40分钟内检测LOD为260 TU/μL的SARS-CoV-2病毒，线性范围为625-10,000 TU/μL。因此，这种方便、快速、灵敏和选择性的检测在分析环境中的SARS-CoV-2病毒颗粒或其他病毒以及监测环境病毒来源方面具有广阔的前景。