ROS-stimulated protein lysine acetylation is required for crown root development in rice,Journal of Advanced Research

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ROS-stimulated protein lysine acetylation is required for crown root development in rice
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-07-29 , DOI: 10.1016/j.jare.2022.07.010
Qiutao Xu ₁ , Yijie Wang ₁ , Zhengting Chen ₁ , Yaping Yue ₁ , Honglin Huang ₁ , Baoguo Wu ₁ , Yuan Liu ₁ , Dao-Xiu Zhou ₂ , Yu Zhao ₁

Affiliation

Introduction

As signal molecules in aerobic organisms, locally accumulated ROS have been reported to balance cell division and differentiation in the root meristem. Protein posttranslational modifications such as lysine acetylation play critical roles in controlling a variety of cellular processes. However, the mechanism by which ROS regulate root development is unknown. In addition, how protein lysine acetylation is regulated and whether cellular ROS levels affect protein lysine acetylation remain unclear.

Objectives

We aimed to elucidate the relationship between ROS and protein acetylation by exploring a rice mutant plant that displays a decreased level of ROS in postembryonic crown root (CR) cells and severe defects in CR development.

Methods

First, proteomic analysis was used to find candidate proteins responsible for the decrease of ROS detected in the wox11 mutant. Then, biochemical, molecular, and genetic analyses were used to study WOX11-regulated genes involved in ROS homeostasis. Finally, acetylproteomic analysis of wild type and wox11 roots treated with or without potassium iodide (KI) and hydrogen peroxide (H₂O₂) was used to study the effects of ROS on protein acetylation in rice CR cells.

Results

We demonstrated that WOX11 was required to maintain ROS homeostasis by upregulating peroxidase genes in the crown root meristem. Acetylproteomic analysis revealed that WOX11-dependent hydrogen peroxide (H₂O₂) levels in CR cells promoted lysine acetylation of many non-histone proteins enriched for nitrogen metabolism and peptide/protein synthesis pathways. Further analysis revealed that the redox state affected histone deacetylases (HDACs) activity, which was likely related to the high levels of protein lysine acetylation in CR cells.

Conclusion

WOX11-controlled ROS level in CR meristem cells is required for protein lysine acetylation which represents a mechanism of ROS-promoted CR development in rice.

中文翻译：

ROS 刺激的蛋白质赖氨酸乙酰化是水稻冠根发育所必需的

介绍

作为需氧生物中的信号分子，据报道局部积累的 ROS 可平衡根分生组织中的细胞分裂和分化。赖氨酸乙酰化等蛋白质翻译后修饰在控制各种细胞过程中起着关键作用。然而，ROS 调节根系发育的机制尚不清楚。此外，蛋白质赖氨酸乙酰化是如何被调控的以及细胞ROS水平是否影响蛋白质赖氨酸乙酰化仍不清楚。

目标

我们旨在通过探索显示胚后冠根 (CR) 细胞中 ROS 水平降低和 CR 发育严重缺陷的水稻突变体植物来阐明 ROS 与蛋白质乙酰化之间的关系。

方法

首先，使用蛋白质组学分析来寻找负责wox11突变体中检测到的 ROS 减少的候选蛋白质。然后，使用生化、分子和遗传分析来研究参与 ROS 稳态的 WOX11 调节基因。最后，使用经或未经碘化钾 (KI) 和过氧化氢 (H ₂ O ₂ ) 处理的野生型和wox11根的乙酰蛋白质组学分析来研究 ROS 对水稻 CR 细胞中蛋白质乙酰化的影响。

结果

我们证明了 WOX11 需要通过上调冠根分生组织中的过氧化物酶基因来维持 ROS 稳态。乙酰蛋白质组学分析表明，CR 细胞中 WOX11 依赖性过氧化氢 (H ₂ O ₂ ) 水平促进了许多非组蛋白的赖氨酸乙酰化，这些非组蛋白富含氮代谢和肽/蛋白质合成途径。进一步分析表明，氧化还原状态影响组蛋白脱乙酰酶 (HDAC) 活性，这可能与 CR 细胞中高水平的蛋白质赖氨酸乙酰化有关。