The European tent caterpillar Malacosoma neustria Linnaeus, 1758 (Lepidoptera: Lasiocampidae), a worldwide pest, feeds on a wide variety of woody and shrub-like plants in its larval stage and causes extensive economic losses. In the fight against this species, environmentally friendly biological control methods should be preferred instead of chemical control. The aim of this study was to evaluate the efficacy of entomopathogenic fungi (EPF) Metarhizium brunneum (ORP-13) and Beauveria bassiana (GOPT-301-2) isolates against the fourth instar larvae of M. neustria under laboratory conditions. M. neustria eggs were collected from the Kızılırmak Delta of Samsun Province, Turkey, and the fourth instar larvae were used in the experiment. Larvae in the control group were fed on sterilized leaves of Eleagnus rhamnoides. Both fungal isolates were applied onto the larvae at 2 ml for each concentration (1 × 106, 1 × 107, and 1 × 108 conidia ml−1). Ten larvae were placed in each group, and sterilized E. rhamnoides leaves were offered to the larvae. The study was carried out in 9 replicates for each group, and the larvae were observed for 14 days. As a result of the study, it was found that the survival rates of the larvae decreased as concentration increased. It was determined that both isolates caused 100% mortality at 1 × 108 conidia ml−1 concentration. The lowest LC50 value was found in larvae exposed to the ORP-13 isolate. It has been suggested that M. brunneum and B. bassiana isolates were virulent for M. neustria larvae and can be used for biological control of this species.

中文翻译：

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Metarhizium brunneum 和 Beauveria bassiana 分离株对欧洲帐篷毛虫 Malacosoma neustria Linnaeus 的功效，1758（鳞翅目：Lasiocampidae）

欧洲帐篷毛虫 Malacosoma neustria Linnaeus, 1758 (Lepidoptera: Lasiocampidae) 是一种世界性害虫，在其幼虫阶段以多种木本和灌木状植物为食，并造成巨大的经济损失。在与该物种的斗争中，应首选环境友好的生物防治方法而不是化学防治。本研究的目的是在实验室条件下评估昆虫病原真菌 (EPF) 绿僵菌 (ORP-13) 和白僵菌 (GOPT-301-2) 分离株对新芽孢杆菌第四龄幼虫的疗效。M. neustria 卵是从土耳其萨姆松省的 Kızılırmak Delta 收集的，并在实验中使用了四龄幼虫。对照组的幼虫喂食已消毒的沙棘叶。对于每个浓度（1 × 106、1 × 107 和 1 × 108 分生孢子 ml-1），将两种真菌分离物以 2 ml 施用于幼虫。每组放置10只幼虫，给幼虫提供无菌沙棘叶。该研究每组重复9次，观察幼虫14天。研究结果发现，幼虫的存活率随着浓度的增加而降低。已确定两种分离株在 1 × 108 分生孢子 ml-1 浓度下导致 100% 的死亡率。在暴露于 ORP-13 分离物的幼虫中发现了最低的 LC50 值。有人提出，M. brunneum 和 B. bassiana 分离株对 M. neustria 幼虫具有毒性，可用于该物种的生物防治。和 1 × 108 分生孢子 ml-1)。每组放置10只幼虫，给幼虫提供无菌沙棘叶。该研究每组重复9次，观察幼虫14天。研究结果发现，幼虫的存活率随着浓度的增加而降低。已确定两种分离株在 1 × 108 分生孢子 ml-1 浓度下导致 100% 的死亡率。在暴露于 ORP-13 分离物的幼虫中发现了最低的 LC50 值。有人提出，M. brunneum 和 B. bassiana 分离株对 M. neustria 幼虫具有毒性，可用于该物种的生物防治。和 1 × 108 分生孢子 ml-1)。每组放置10只幼虫，给幼虫提供无菌沙棘叶。该研究每组重复9次，观察幼虫14天。研究结果发现，幼虫的存活率随着浓度的增加而降低。已确定两种分离株在 1 × 108 分生孢子 ml-1 浓度下导致 100% 的死亡率。在暴露于 ORP-13 分离物的幼虫中发现了最低的 LC50 值。有人提出，M. brunneum 和 B. bassiana 分离株对 M. neustria 幼虫具有毒性，可用于该物种的生物防治。观察幼虫14天。研究结果发现，幼虫的存活率随着浓度的增加而降低。已确定两种分离株在 1 × 108 分生孢子 ml-1 浓度下导致 100% 的死亡率。在暴露于 ORP-13 分离物的幼虫中发现了最低的 LC50 值。有人提出，M. brunneum 和 B. bassiana 分离株对 M. neustria 幼虫具有毒性，可用于该物种的生物防治。观察幼虫14天。研究结果发现，幼虫的存活率随着浓度的增加而降低。已确定两种分离株在 1 × 108 分生孢子 ml-1 浓度下导致 100% 的死亡率。在暴露于 ORP-13 分离物的幼虫中发现了最低的 LC50 值。有人提出，M. brunneum 和 B. bassiana 分离株对 M. neustria 幼虫具有毒性，可用于该物种的生物防治。