Sleep is a necessity for our survival, but its regulation remains incompletely understood. Here, we used a human sleep duration gene to identify a population of cells in the peri-tegmental reticular nucleus (pTRN ADRB1 ) that regulate sleep–wake, uncovering a role for a poorly understood brain area. Although initial ablation in mice led to increased wakefulness, further validation revealed that pTRN ADRB1 neuron stimulation strongly promotes wakefulness, even after stimulation offset. Using combinatorial genetics, we found that excitatory pTRN ADRB1 neurons promote wakefulness. pTRN neurons can be characterized as anterior- or posterior-projecting neurons based on multiplexed analysis of projections by sequencing (MAPseq) analysis. Finally, we found that pTRN ADRB1 neurons promote wakefulness, in part, through projections to the lateral hypothalamus. Thus, human genetic information from a human sleep trait allowed us to identify a role for the pTRN in sleep–wake regulation.

中文翻译：

---

用于维持唤醒的兴奋性被盖周围网状核回路


睡眠是我们生存的必需品，但其调节机制仍不完全清楚。在这里，我们使用人类睡眠持续时间基因来识别被盖周围网状核（pTRN）中的细胞群ADRB1 ）调节睡眠-觉醒，揭示了人们知之甚少的大脑区域的作用。尽管最初的消融会导致小鼠的觉醒程度增加，但进一步的验证表明 pTRN ADRB1即使在刺激抵消后，神经元刺激也会强烈促进觉醒。使用组合遗传学，我们发现兴奋性 pTRN ADRB1神经元促进觉醒。根据测序投影多重分析 (MAPseq)，pTRN 神经元可被表征为前投影神经元或后投影神经元。最后我们发现pTRN ADRB1神经元部分地通过投射到下丘脑外侧来促进觉醒。因此，来自人类睡眠特征的人类遗传信息使我们能够确定 pTRN 在睡眠-觉醒调节中的作用。