Feco-orally transmitted infectious diseases are common in Nigeria where the potable water access is poor. In the south-western Nigerian Ibadan metropolis, supply of municipal water is meagre as residents depend on household wells and boreholes. The likelihood of fecal contamination of household water sources in Ibadan was examined longitudinally to quantify and understand its impact. Well and borehole water samples aseptically collected from 96 households in Ibadan were assessed for total heterotrophic counts (THCs), total coliform counts (TCCs) and total Escherichia coli counts (TECs) using a pour plate technique. E. coli were identified by uidA and whole-genome sequencing using Illumina technology, whereas virulence factors were predicted using VirulenceFinder. There was season-independent abundance of THC and TCC in the well and borehole with a significant recovery of E. coli in the wells during the wet season compared to the dry season (P = 0.0001). Virulence genes associated with pathogenic E. coli were identified in 13 (52%) strains with one E. coli each classified as extra-intestinal E. coli, avian pathogenic E. coli and enteroaggregative E. coli. High heterotrophic and coliform counts, with rainfall-driven E. coli contamination revealed that the water sources evaluated in this study are unfit for consumption.

粪口传播的传染病在尼日利亚很常见，那里的饮用水供应很差。在尼日利亚西南部的伊巴丹大都市，由于居民依赖家庭水井和钻孔，市政供水不足。对伊巴丹家庭水源受到粪便污染的可能性进行了纵向检查，以量化和了解其影响。使用倒板技术评估从伊巴丹 96 个家庭无菌采集的井水和井水样本的异养菌总数 (THC)、大肠菌群总数 (TCC) 和大肠杆菌总数 (TEC)。大肠杆菌由uidA鉴定和使用 Illumina 技术的全基因组测序，而使用 VirulenceFinder 预测毒力因子。井和钻孔中的 THC 和 TCC 丰度与季节无关，与旱季相比，雨季井中的大肠杆菌显着恢复( P = 0.0001)。在 13 个 (52%) 菌株中鉴定出与致病性大肠杆菌相关的毒力基因，每个菌株中有一种大肠杆菌被归类为肠外大肠杆菌、禽类致病性大肠杆菌和肠聚集性大肠杆菌。高异养和大肠菌群计数，降雨驱动的大肠杆菌污染表明本研究中评估的水源不适合饮用。