Triacylglycerols (TAGs) containing positional isomers of hypogeic (Hy), palmitoleic (Po), and palmitvaccenic (Pv) acids from three microorganisms (top-fermenting brewer's yeast Saccharomyces cerevisiae, green alga Coccomyxa elongata, and arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis) were analyzed. Dozens of regioisomers and enantiomers of TAGs containing one, two or three hexadecenoic acids have been identified by means of reversed phase chromatography/mass spectrometry (RP-HPLC/MS). The regioisomers of TAGs containing two palmitic acids and any hexadecenoic acid were separated. Analysis of regioisomers of TAGs having one Pv residue showed that asymmetric molecular species such as PvPP or PPPv were dominant in Rhizophagus. TAGs were also analyzed on a chiral phase column and nine molecular species of TAGs containing two palmitic and any of three hexadecenoic acids were separated and identified. In the case of TAGs containing one palmitic and two hexadecenoic acids, the separation was successful only if the hexadecenoic acids were identical. Separation of TAGs containing three hexadecenoic acids was successful only if all three hexadecenoic acids were identical. Regardless of the type of TAG, it was found that TAGs in the AM fungus and containing palmitvaccenic acid bound at the sn-1 position of the glycerol backbone were dominant, suggesting similarity in the biosynthesis of the different TAGs. The covalent adduct chemical ionization method was used for identification of TAGs as adduct with (1-methyleneimino)-1-ethenyl ion, which reacted with double bond of the unsaturated fatty acid. Tandem MS thus makes it possible to identify TAGs containing various hexadecenoic acids.

三酰基甘油 (TAG) 含有来自三种微生物（顶部发酵啤酒酵母Saccharomyces cerevisiae、绿藻Coccomyxa elongata和丛枝菌根 (AM) 真菌Rhizophagus 不规则菌）的 Hypogeic (Hy)、palmitoleic (Po) 和 palmitvaccenic (Pv) 酸的位置异构体) 进行了分析。已通过反相色谱/质谱 (RP-HPLC/MS) 鉴定了数十种含有一种、两种或三种十六碳烯酸的 TAG 区域异构体和对映异构体。分离含有两种棕榈酸和任何十六碳烯酸的TAG的区域异构体。对具有一个 Pv 残基的 TAG 区域异构体的分析表明，PvPP 或 PPPv 等不对称分子种类在根霉属中占优势。. 还在手性相柱上分析了 TAG，分离并鉴定了含有两种棕榈酸和三种十六碳烯酸中的任何一种的九种 TAG 分子种类。在含有一种棕榈酸和两种十六碳烯酸的 TAG 的情况下，只有当十六碳烯酸相同时，分离才会成功。仅当所有三种十六碳烯酸相同时，才能成功分离含有三种十六碳烯酸的 TAG。无论 TAG 的类型如何，发现 AM 真菌中的 TAGs 和含有棕榈酸甘油骨架的-1位置占主导地位，表明不同TAG的生物合成相似。采用共价加合物化学电离法鉴定TAG为与(1-亚甲基亚氨基)-1-乙烯基离子的加合物，与不饱和脂肪酸的双键反应。因此，串联 MS 可以识别含有各种十六碳烯酸的 TAG。