Characterization of Tremulane Sesquiterpene Synthase from the Basidiomycete Irpex lacteus,Organic Letters

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Characterization of Tremulane Sesquiterpene Synthase from the Basidiomycete Irpex lacteus
Organic Letters ( IF 4.9 ) Pub Date : 2022-07-29 , DOI: 10.1021/acs.orglett.2c01975
Rong Chen _{1,

2} , Tao Feng ₃ , Min Li ₄ , Xinyi Zhang ₄ , Juan He ₃ , Ben Hu ₅ , Zixin Deng _{1,

4} , Tiangang Liu _{1,

4} , Ji-Kai Liu ₃ , Xinghuan Wang _{1,

2,

6} , Guangkai Bian _{1,

4,

6}

Affiliation

Tremulane sesquiterpenoids are key secondary metabolites of the basidiomycete Irpex lacteus, which displays structural diversity and various bioactivities. However, tremulane sesquiterpene synthases have not been reported to date. The tremulane sesquiterpene synthase of I. lacteus was characterized by genome mining, heterologous expression, an in vitro assay, and substrate feeding. Moreover, the structures of the corresponding products were elucidated by NMR spectroscopy and X-ray diffraction analysis.

中文翻译：

担子菌 Irpex lacteus 的 Tremulane 倍半萜合酶的表征

Tremulane 倍半萜类化合物是担子菌Irpex lacteus的关键次级代谢产物，具有结构多样性和多种生物活性。然而，迄今为止尚未报道海藻烷倍半萜合酶。I. lacteus的海藻烷倍半萜合酶通过基因组挖掘、异源表达、体外测定和底物喂养来表征。此外，通过NMR光谱和X射线衍射分析阐明了相应产物的结构。

更新日期：2022-07-29

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