Herbivore-induced plant volatiles (HIPVs) help tea plant (Camellia sinensis) adapt to environmental stress, and they are also quality-related components of tea. However, the upstream mechanism regulating the herbivore-induced expression of volatile biosynthesis genes is unclear, especially at the level of epigenetic regulation. In this study, similar to the effects of a tea green leafhopper infestation, treatments with exogenous jasmonic acid (JA) and histone deacetylase inhibitors significantly increased the (E)-nerolidol content in tea and induced the expression of the associated biosynthesis gene CsNES. Furthermore, a key transcription factor related to JA signaling, myelocytomatosis 2 (CsMYC2), interacted with the histone deacetylase 2 (CsHDA2) in vitro and in vivo. A tea green leafhopper infestation inhibited CsHDA2 expression and decreased CsHDA2 abundance. Moreover, the tea green leafhopper infestation increased the H3 and H4 acetylation levels in the promoter region of CsNES, which in turn up-regulated the expression of CsNES and increased the (E)-nerolidol content. In this study, we revealed the effects of histone acetylations on the accumulation of HIPVs, while also confirming that CsHDA2-CsMYC2 is an important transcriptional regulatory module for the accumulation of (E)-nerolidol induced by tea green leafhoppers. The results of this study may be useful for characterizing plant aromatic compounds and the main upstream stress-responsive signaling molecules. Furthermore, the study findings will assist researchers clarify the epigenetic regulation influencing plant secondary metabolism in response to external stress.

中文翻译：

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组蛋白去乙酰化酶 CsHDA2 参与调节暴露于茶绿叶蝉侵染的茶 (Camellia sinensis) 中 (E)-橙花醇的形成

草食动物诱导的植物挥发物 (HIPVs) 有助于茶树 (Camellia sinensis) 适应环境压力，它们也是茶叶的质量相关成分。然而，调控草食动物诱导的挥发性生物合成基因表达的上游机制尚不清楚，尤其是在表观遗传调控水平。在本研究中，与茶绿叶蝉侵染的影响相似，外源茉莉酸 (JA) 和组蛋白脱乙酰酶抑制剂处理显着增加了茶中 (E)-橙花醇的含量，并诱导了相关生物合成基因 CsNES 的表达。此外，与 JA 信号传导相关的关键转录因子髓细胞瘤病 2 (CsMYC2) 在体外和体内与组蛋白脱乙酰酶 2 (CsHDA2) 相互作用。茶绿叶蝉侵染抑制 CsHDA2 表达并降低 CsHDA2 丰度。此外，茶绿叶蝉侵染增加了CsNES启动子区域的H3和H4乙酰化水平，进而上调了CsNES的表达，增加了(E)-橙花油的含量。在这项研究中，我们揭示了组蛋白乙酰化对 HIPVs 积累的影响，同时也证实了 CsHDA2-CsMYC2 是茶绿叶蝉诱导的 (E)-nerolidol 积累的重要转录调控模块。这项研究的结果可能有助于表征植物芳香化合物和主要的上游应激反应信号分子。此外，