Pesticide pollution has become a severe challenge for harming food quality and human safety. Therefore, it’s urgent to develop sensitive methods to detect and analyse pesticides. Herein, we successfully developed a new turn-on fluorescent chemosensor (TPE-4P), a tetraphenyl ethylene derivative modified with four pillar[5]arenes, which could sensitively, selectively, and rapidly detect the pesticide paraquat. In our study, we took full advantage of both the host-guest strategy and aggregation-induced emission (AIE) to achieve fluorescent enhancement of the chemosensor for paraquat, in which four pillar[5]arenes as the recognition units can selectively capture paraquat. The formation of the supramolecular complexes based on the interlaced host-guest interactions effectively restricted the intramolecular single-bond rotation and skeletal vibration, resulting in the intensive fluorescent emission. The detection limit for paraquat was low enough to reach 154.1 nM, and the detection time was short enough to meet the rapid detection conditions. Moreover, the test strips loaded-TPE-4P facilitated the detection process and achieved the semi-quantitative analysis for paraquat in real spiked samples. In addition, TPE-4P could respond to paraquat in living zebrafish obviously. These results suggest that the cooperation of host-guest and AIE chemistry could become useful for the design to detect some appropriate organic molecules.

农药污染已成为危害食品质量和人身安全的严峻挑战。因此，迫切需要开发灵敏的方法来检测和分析农药。在此，我们成功开发了一种新型开启荧光化学传感器（TPE-4P)，一种四柱[5]芳烃修饰的四苯基乙烯衍生物，可灵敏、选择性、快速地检测农药百草枯。在我们的研究中，我们充分利用主客体策略和聚集诱导发射（AIE）来实现百草枯化学传感器的荧光增强，其中四个柱[5]芳烃作为识别单元可以选择性地捕获百草枯。基于交错的主客体相互作用的超分子复合物的形成有效地限制了分子内单键旋转和骨架振动，导致强烈的荧光发射。百草枯的检测限低至154.1 nM，检测时间短，可满足快速检测条件。此外，加载的试纸——TPE-4P促进了检测过程，实现了实际加标样品中百草枯的半定量分析。此外，TPE-4P对活斑马鱼体内的百草枯有明显的反应。这些结果表明，主客体和 AIE 化学的合作可能有助于设计检测一些合适的有机分子。