Electric-Field-Mediated In-Sensor Alignment of Antibody’s Orientation to Enhance the Antibody–Antigen Binding for Ultrahigh Sensitivity Sensors,Nano Letters

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Electric-Field-Mediated In-Sensor Alignment of Antibody’s Orientation to Enhance the Antibody–Antigen Binding for Ultrahigh Sensitivity Sensors
Nano Letters ( IF 9.6 ) Pub Date : 2022-07-28 , DOI: 10.1021/acs.nanolett.2c01584
Hye Jin Kim _{1,

2} , Dongsung Park ₃ , Yejin Park ₄ , Dae-Hyeong Kim _{1,

5} , Jinsik Kim ₄

Affiliation

Applying an electric-field (E-field) during antibody immobilization aligns the orientation of the antibody on the biosensor surface, thereby enhancing the binding probability between the antibody and antigen and maximizing the sensitivity of the biosensor. In this study, a biosensor with enhanced antibody–antigen binding probability was developed using the alignment of polar antibodies (immunoglobulin G [IgG]) under an E-field applied inside the interdigitated electrodes. The optimal alignment condition was first theoretically calculated and then experimentally confirmed by comparing the impedance change before and after the alignment of IgG (a purified anti-β-amyloid antibody). With the optimized condition, the impedance change of the biosensor was maximized because of the alignment of IgG orientation on the sensor surface; the detection sensitivity of the antigen amyloid-beta 1–42 was also maximized. The E-field-based in-sensor alignment of antibodies is an easy and effective method for enhancing biosensor sensitivity.

中文翻译：

电场介导的抗体方向的传感器内对齐以增强超高灵敏度传感器的抗体-抗原结合

在抗体固定期间施加电场（E场）使抗体在生物传感器表面上的方向对齐，从而提高抗体与抗原之间的结合概率，并使生物传感器的灵敏度最大化。在这项研究中，使用极性抗体（免疫球蛋白 G [IgG]）在E- 施加在叉指电极内部的场。首先从理论上计算出最佳比对条件，然后通过比较IgG（一种纯化的抗β-淀粉样蛋白抗体）比对前后的阻抗变化进行实验确认。在优化条件下，由于传感器表面IgG取向的对齐，生物传感器的阻抗变化最大化；抗原β-淀粉样蛋白1-42的检测灵敏度也最大化。基于电场的抗体传感器内对齐是提高生物传感器灵敏度的一种简单有效的方法。

更新日期：2022-07-28

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