Abstract

Cyanobacteria are photosynthetic aquatic microorganisms with light and dark photosynthesis reactions. In the present study, the effect of various light spectrums on light and dark reactions in Spirulina platensis was investigated. Chlorophyll a and phycocyanin as light reaction pigments and vitamin B₁₂ as a product of dark reaction were examined. S. platensis was exposed to four different color LEDs (white, red, blue, and yellow) at a fixed intensity. In light reaction, chlorophyll-a has the highest amount in blue, red, white, and yellow by 61.23, 45.46, 34.85, and 22.55 mg·g⁻¹, respectively. Those microalgae cultured in the blue color produced the highest amount of Chlorophyll a and c-phycocyanin. For C-phycocyanin, the highest to the lowest amount belong to blue, red, yellow, and white light by 168.6, 102.4, 85.77, and 53.81 mg·g⁻¹. The amount of vitamin B₁₂, from high to low is related to blue, red, white, and yellow light by 68.91, 59.73, 52.56, and 10.2 µg·g⁻¹. The trend of vitamin B₁₂’s production was similar to the chlorophyll a. In conclusion, blue color can increase the production of vitamin B₁₂ while keeping light reaction products at their highest level.

摘要

蓝藻是光合水生微生物，具有光和暗光合作用反应。在本研究中，研究了不同光谱对钝顶螺旋藻中光反应和暗反应的影响。检测了作为光反应色素的叶绿素a和藻蓝蛋白以及作为暗反应产物的维生素B _{12 。}S. platensis以固定强度暴露于四种不同颜色的 LED（白色、红色、蓝色和黄色）。在光反应中，叶绿素-a在蓝色、红色、白色和黄色中含量最高，分别为61.23、45.46、34.85和22.55 mg·g ^-1。那些以蓝色培养的微藻产生的叶绿素a含量最高和c-藻蓝蛋白。对于C-藻蓝蛋白，从最高到最低分别为168.6、102.4、85.77和53.81 mg·g -1 的蓝光、红光、黄光和^白光。_{维生素B 12}含量从高到低与蓝光、红光、白光和黄光的相关性分别为68.91、59.73、52.56和10.2 µg·g ^-1。_{维生素B 12}的产生趋势与叶绿素a相似。_{总之，蓝色可以增加维生素 B 12}的产量，同时将光反应产物保持在最高水平。