Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 µmol·L⁻¹, significantly lower than that of the threshold value of 0.312 µmol·L⁻¹, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 µmol·L⁻¹). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

肼具有剧毒，对人体造成严重伤害。在此，以天然肉桂醛为起始材料，合成了一种新型荧光探针 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT)，用于检测肼。与各种常见的金属离子、阴离子和胺化合物相比，该探针对肼的荧光响应显着增强。探针FHT对肼的检测限低至0.14 µmol·L ^-1，明显低于环保局规定的阈值0.312 µmol·L ^{-1 。}此外，所提出的探针能够在宽 pH（5-10）和线性检测范围（0-110 µmol·L ^-1）。该探针用于测定不同环境水样中的痕量肼。装载探针FHT的滤纸条能够通过明显的肉眼和荧光颜色变化方便地检测低浓度的肼。重要的是，具有低细胞毒性的探针FHT已成功应用于活体 Hela 细胞和斑马鱼中的肼可视化。