Cell mechanotransduction plays an important role in vascular regulation and disease development. Excessive accumulation of ROS, especially superoxide anion radicals (O₂˙⁻), is closely related to cardiovascular diseases. Lately, NADPH oxidases, which are the major source of O₂˙⁻ production in vascular tissues, have been demonstrated to be involved in cardiovascular diseases. Therefore, in situ and real-time monitoring of superoxide anions (O₂˙⁻) is essential for exploring the mechanisms of mechanotransduction associated with NADPH oxidase function in living cells. Here we report a rationally designed ultrasonication-assisted approach for growing Au nanoflower films on a flexible surface, which serves as the desired interface for cysteine and superoxide dismutase (SOD) anchoring to form a flexible and stretchable electrode (SOD/Cys/Au SE). The SOD/Cys/Au SE shows good stretchability, fast electron-transfer rates, and high selectivity to measure O₂˙⁻ released from cells during the stretching states. Our strategy provides a basis for developing more sophisticated stretchable biosensing tools to induce and monitor transient biochemical signals during cell mechanotransduction.

中文翻译：

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用于原位监测细胞力学转导中超氧阴离子释放的基于金纳米花薄膜的可拉伸生物传感器

细胞机械转导在血管调节和疾病发展中起重要作用。_{ROS，尤其是超氧阴离子自由基（O 2} ˙ ^- ）的过度积累与心血管疾病密切相关。_{最近，作为血管组织中 O 2} ˙ ^-产生的主要来源的 NADPH 氧化酶已被证明与心血管疾病有关。因此，超氧阴离子（O ₂ ˙ ^-) 对于探索与活细胞中 NADPH 氧化酶功能相关的机械转导机制至关重要。在这里，我们报告了一种合理设计的超声辅助方法，用于在柔性表面上生长 Au 纳米花膜，该方法可作为半胱氨酸和超氧化物歧化酶 (SOD) 锚定以形成柔性和可拉伸电极 (SOD/Cys/Au SE) 的理想界面. SOD/Cys/Au SE 表现出良好的可拉伸性、快速的电子转移速率和高选择性以测量在拉伸状态下从细胞中释放的O ₂ ˙ ^{- 。}我们的策略为开发更复杂的可拉伸生物传感工具以在细胞机械转导过程中诱导和监测瞬态生化信号提供了基础。