Improved production of heme using metabolically engineered Escherichia coli,Biotechnology and Bioengineering

当前位置： X-MOL 学术 › Biotechnol. Bioeng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Improved production of heme using metabolically engineered Escherichia coli
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-07-27 , DOI: 10.1002/bit.28194
Kyeong Rok Choi _{1,

2} , Hye Eun Yu ₁ , Hoseong Lee ₁ , Sang Yup Lee _{1,

2,

3}

Affiliation

Heme has recently attracted much attention due to its promising applications in the food and healthcare industries. However, the current titers and productivities of heme produced by recombinant microorganisms are not high enough for a wide range of applications. In this study, the process for the fermentation of the metabolically engineered Escherichia coli HAEM7 strain was optimized for the high-level production of heme. To improve the production of heme, different carbon sources, iron concentration in the medium, pH control strategies, induction points, and iron content in the feeding solution were examined. Moreover, strategies of increasing cell density, regular iron supplementation, and supply of excess feeding solution were developed to further improve the production of heme. In the optimized fermentation process, the HAEM7 strain produced 1.03 g/L heme with productivity of 21.5 mg/L/h. The fermentation process and strategies reported here will expedite establishing industry-level production of heme.

中文翻译：

使用代谢工程大肠杆菌改进血红素的生产

血红素最近因其在食品和医疗保健行业的应用前景而备受关注。然而，目前由重组微生物产生的血红素滴度和产率对于广泛的应用来说还不够高。在这项研究中，代谢工程大肠杆菌的发酵过程HAEM7 菌株针对血红素的高水平生产进行了优化。为了提高血红素的产生，检查了不同的碳源、培养基中的铁浓度、pH 控制策略、诱导点和补料溶液中的铁含量。此外，还制定了增加细胞密度、定期补充铁和供应过量喂养液的策略，以进一步提高血红素的产生。在优化的发酵过程中，HAEM7菌株产生1.03 g/L的血红素，生产率为21.5 mg/L/h。这里报告的发酵过程和策略将加快建立工业级血红素生产。

更新日期：2022-07-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11