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A Drop-in, Focus-Extending Phase Mask Simplifies Microscopic and Microfluidic Imaging Systems for Cost-Effective Point-of-Care Diagnostics
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-07-27 , DOI: 10.1021/acs.analchem.2c01421 Qi Meng 1 , Yaning Li 1 , Yajun Yu 1 , Kaiqin Chu 2 , Zachary J Smith 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-07-27 , DOI: 10.1021/acs.analchem.2c01421 Qi Meng 1 , Yaning Li 1 , Yajun Yu 1 , Kaiqin Chu 2 , Zachary J Smith 1
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Microscopic imaging and imaging flow cytometry have wide potential in point-of-care assays; however, their narrow depth of focus necessitates precise mechanical or fluidic focus control of a sample in order to acquire high-quality images that can be used for downstream analysis, increasing the cost and complexity of the imaging system. This complexity represents a barrier to miniaturization and translation of point-of-care assays based on microscopic imaging or imaging flow cytometry. To address this challenge, we present a simple drop-in phase mask with a physics-informed, circularly symmetric asphere phase profile that extends the depth of focus by >5-fold while largely preserving the image quality compared to other depth extending methods. We show that such a focus-extended system overcomes manufacturing tolerances in low-cost sample chambers, enlarges the useable field-of-view of low-cost objectives, and permits increased throughput and precision in flow imaging systems without the need for complex flow-focusing. As the image quality is preserved without the need for postacquisition image restoration, our solution is also highly appropriate for on-line applications such as cell sorting.
中文翻译：
插入式、扩展焦点的相位掩模简化了显微和微流体成像系统，用于经济高效的床旁诊断
显微成像和成像流式细胞术在即时检测中具有广泛的潜力；然而，它们狭窄的焦深需要对样品进行精确的机械或流体聚焦控制，以获得可用于下游分析的高质量图像，从而增加了成像系统的成本和复杂性。这种复杂性代表了基于显微成像或成像流式细胞术的即时检测的小型化和转化的障碍。为了应对这一挑战，我们提出了一个简单的插入式相位掩模，它具有物理信息的圆形对称非球面相位轮廓，与其他深度扩展方法相比，它可以将焦深扩展 > 5 倍，同时在很大程度上保持图像质量。我们表明，这种焦点扩展系统克服了低成本样品室的制造公差，扩大了低成本物镜的可用视野，并允许增加流动成像系统的吞吐量和精度，而无需复杂的流动-聚焦。由于无需进行采集后图像恢复即可保留图像质量，因此我们的解决方案也非常适用于细胞分选等在线应用。
更新日期：2022-07-27
中文翻译：
插入式、扩展焦点的相位掩模简化了显微和微流体成像系统，用于经济高效的床旁诊断
显微成像和成像流式细胞术在即时检测中具有广泛的潜力；然而，它们狭窄的焦深需要对样品进行精确的机械或流体聚焦控制，以获得可用于下游分析的高质量图像，从而增加了成像系统的成本和复杂性。这种复杂性代表了基于显微成像或成像流式细胞术的即时检测的小型化和转化的障碍。为了应对这一挑战，我们提出了一个简单的插入式相位掩模，它具有物理信息的圆形对称非球面相位轮廓，与其他深度扩展方法相比，它可以将焦深扩展 > 5 倍，同时在很大程度上保持图像质量。我们表明，这种焦点扩展系统克服了低成本样品室的制造公差，扩大了低成本物镜的可用视野，并允许增加流动成像系统的吞吐量和精度，而无需复杂的流动-聚焦。由于无需进行采集后图像恢复即可保留图像质量，因此我们的解决方案也非常适用于细胞分选等在线应用。
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