BnaPPT1 is essential for chloroplast development and seed oil accumulation in Brassica napus,Journal of Advanced Research

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BnaPPT1 is essential for chloroplast development and seed oil accumulation in Brassica napus
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-07-27 , DOI: 10.1016/j.jare.2022.07.008
Shan Tang ₁ , Fei Peng ₁ , Qingqing Tang ₁ , Yunhao Liu ₁ , Hui Xia ₁ , Xuan Yao ₁ , Shaoping Lu ₁ , Liang Guo ₁

Affiliation

Introduction

Phosphoenolpyruvate/phosphate translocator (PPT) transports phosphoenolpyruvate from the cytosol into the plastid for fatty acid (FA) and other metabolites biosynthesis.

Objectives

This study investigated PPTs’ functions in plant growth and seed oil biosynthesis in oilseed crop Brassica napus.

Methods

We created over-expression and mutant material of BnaPPT1. The plant development, oil content, lipids, metabolites and ultrastructure of seeds were compared to evaluate the gene function.

Results

The plastid membrane localized BnaPPT1 was found to be required for normal growth of B. napus. The plants grew slower with yellowish leaves in BnaA08.PPT1 and BnaC08.PPT1 double mutant plants. The results of chloroplast ultrastructural observation and lipid analysis show that BnaPPT1 plays an essential role in membrane lipid synthesis and chloroplast development in leaves, thereby affecting photosynthesis. Moreover, the analysis of primary metabolites and lipids in developing seeds showed that BnaPPT1 could impact seed glycolytic metabolism and lipid level. Knockout of BnaA08.PPT1 and BnaC08.PPT1 resulted in decreasing of the seed oil content by 2.2 to 9.1%, while overexpression of BnaC08.PPT1 significantly promoted the seed oil content by 2.1 to 3.3%.

Conclusion

Our results suggest that BnaPPT1 is necessary for plant chloroplast development, and it plays an important role in maintaining plant growth and promoting seed oil accumulation in B. napus.

中文翻译：

BnaPPT1 对欧洲油菜叶绿体发育和种子油积累至关重要

介绍

磷酸烯醇丙酮酸/磷酸转运蛋白 (PPT) 将磷酸烯醇丙酮酸从胞质溶胶转运到质体中，用于脂肪酸 (FA) 和其他代谢物的生物合成。

目标

本研究调查了 PPT 在油料作物 Brassica napus 的植物生长和种子油生物合成中的功能。

方法

我们创建了 BnaPPT1 的过表达和突变材料。比较种子的植物发育、含油量、脂质、代谢物和超微结构以评价基因功能。

结果

发现质体膜定位的 BnaPPT1 是欧洲油菜正常生长所必需的。BnaA08.PPT1和BnaC08.PPT1双突变体植株生长较慢，叶片呈黄色。叶绿体超微结构观察和脂质分析结果表明，BnaPPT1在叶片膜脂合成和叶绿体发育中发挥重要作用，从而影响光合作用。此外，对发育种子中初级代谢物和脂质的分析表明，BnaPPT1 可以影响种子糖酵解代谢和脂质水平。敲除 BnaA08.PPT1 和 BnaC08.PPT1 导致种子含油量降低 2.2% 至 9.1%，而 BnaC08.PPT1 的过表达显着提高种子含油量 2.1% 至 3.3%。