With the rapid development of physiopathology and the surge in demand for comprehension of micro-scale physiological events, AIE-based bio-probes are found superior in presenting precise and practical results in enzyme imaging and analysis with a high signal-to-noise ratio and non-destructive operation. By delivering enzyme-responding “light-up” fluorescence signals, the visual and real-time tracking of the distribution and activity of intracellular enzymes is accomplished with AIE-based bio-probes. In particular, by combining with modern nano-encapsulation technologies, AIE-based compounds can realize the simultaneous diagnosis and treatment of specific diseases that are difficult to deal with through traditional strategies. This review summarizes and generalizes the typical AIE-based bio-probes reported recently based on the AIE mechanisms of solubility changes, excited-state intramolecular proton transfer (ESIPT), electrostatic interactions, and hydrophobic interactions, expounding their great values in the bio-sensing and bio-medicine field. Advanced enzyme detection and estimation, cell identification, disease diagnosis, and controlled drug release are demonstrated with high confidence and reproducibility. Through the in-depth analysis of these bio-probes’ design and working principles, currently existing drawbacks and further future directions are subsequently proposed to promote a more prosperous development of AIE-based enzyme probes.

中文翻译：

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当 AIE 遇上酶

随着病理生理学的快速发展和对微尺度生理事件理解需求的激增，基于AIE的生物探针在酶成像和分析中表现出精确和实用的结果，具有高信噪比和无损操作。通过提供酶响应“点亮”荧光信号，使用基于 AIE 的生物探针实现对细胞内酶分布和活性的可视化和实时跟踪。特别是结合现代纳米封装技术，基于AIE的化合物可以实现传统策略难以应对的特定疾病的同步诊断和治疗。本文从溶解度变化、激发态分子内质子转移（ESIPT）、静电相互作用和疏水相互作用等AIE机制对近期报道的典型AIE生物探针进行了总结和概括，阐述了它们在生物传感中的重要价值。和生物医药领域。先进的酶检测和估计、细胞鉴定、疾病诊断和受控药物释放都以高置信度和可重复性得到证明。通过对这些生物探针的设计和工作原理的深入分析，随后提出了目前存在的缺陷和未来的进一步发展方向，以促进基于AIE的酶探针的更加繁荣的发展。阐述了他们在生物传感和生物医药领域的巨大价值。先进的酶检测和估计、细胞鉴定、疾病诊断和受控药物释放都以高置信度和可重复性得到证明。通过对这些生物探针的设计和工作原理的深入分析，随后提出了目前存在的缺陷和未来的进一步发展方向，以促进基于AIE的酶探针的更加繁荣的发展。阐述了他们在生物传感和生物医药领域的巨大价值。先进的酶检测和估计、细胞鉴定、疾病诊断和受控药物释放都以高置信度和可重复性得到证明。通过对这些生物探针的设计和工作原理的深入分析，随后提出了目前存在的缺陷和未来的进一步发展方向，以促进基于AIE的酶探针的更加繁荣的发展。