Production of zinc protoporphyrin IX by metabolically engineered Escherichia coli,Biotechnology and Bioengineering

当前位置： X-MOL 学术 › Biotechnol. Bioeng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Production of zinc protoporphyrin IX by metabolically engineered Escherichia coli
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-07-26 , DOI: 10.1002/bit.28195
Kyeong Rok Choi _{1,

2} , Hye Eun Yu ₁ , Sang Yup Lee _{1,

2,

3}

Affiliation

Zinc protoporphyrin IX (ZnPPIX) has been considered as a promising red colorant for food industries as well as an anticancer drug. However, bio-based production of ZnPPIX from a renewable carbon source has not been reported yet. In this study, a fermentation process of the metabolically engineered Escherichia coli HAEM7 strain was optimized for the high-level production of ZnPPIX. To repurpose the HAEM7 strain that was originally developed for the production of heme into a producer of ZnPPIX, the concentrations of iron and zinc in the culture medium were rebalanced. Next, the concentration of zinc in the feeding solution was optimized to improve ZnPPIX production. Moreover, the pH control strategy, induction point, and the strategy of increasing the cell density, which were optimized in the accompanying paper for the high-level production of heme, were applied together. In the optimized fermentation process, the HAEM7 strain produced 2.2 g/L ZnPPIX with a productivity of 39.9 mg/L/h. The fermentation process and strategies reported here will expedite establishing industry-level production of ZnPPIX.

中文翻译：

代谢工程大肠杆菌生产锌原卟啉 IX

锌原卟啉 IX (ZnPPIX) 已被认为是一种用于食品工业的有前途的红色着色剂以及一种抗癌药物。然而，尚未报道从可再生碳源生物基生产 ZnPPIX。在这项研究中，代谢工程大肠杆菌的发酵过程HAEM7 菌株针对 ZnPPIX 的高水平生产进行了优化。为了将最初开发用于生产血红素的 HAEM7 菌株重新用于生产 ZnPPIX，重新平衡了培养基中铁和锌的浓度。接下来，优化补料溶液中锌的浓度以提高 ZnPPIX 的产量。此外，将随附论文中针对血红素的高水平生产优化的 pH 控制策略、诱导点和增加细胞密度的策略一起应用。在优化的发酵过程中，HAEM7菌株产2.2 g/L ZnPPIX，产率为39.9 mg/L/h。这里报告的发酵过程和策略将加快建立工业级生产 ZnPPIX。

更新日期：2022-07-26

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11