The potential of microalgae in wastewater treatment and resource utilization has received considerable interest. For better outcomes in large-scale cultivations, it is usually essential to optimize the microalgal culture parameters in a sub-pilot cultivation system. Herein, a series of experiments were carried out to investigate the effects of photoperiod, CO₂ concentration and aeration intensity on nutrients removal and biomass production of Tetradesmus dimorphus under the 50 L culture scale. The results showed that the removal rates of chemical oxygen demand (CODcr), NH₄⁺-N and total phosphorus (TP) in primary effluent (PE) were 93.9 %, 92.3 % and 84.8 %, respectively. Compared with 15 h:9 h (15 h light and 9 h dark cycle), the maximum Chlorophyll-a content of 2797.78 mg/m³ was reached at 24 h:0 h. The maximum microalgae biomass (OD₆₈₀ = 0.56) was obtained when the CO₂ concentration was 200 mL/min, but CO₂ enrichment inhibited nitrogen removal. At optimal aeration intensity (60 L/h), the highest chlorophyll-a content was recorded on day 3. The removal rates of NH₄⁺-N and TP were 92.1 % and 89.7 %, respectively.

微藻在废水处理和资源利用方面的潜力受到了广泛关注。为了在大规模培养中获得更好的结果，通常必须优化子试点培养系统中的微藻培养参数。在此，开展了一系列实验，研究了光周期、CO ₂浓度和曝气强度对50 L培养规模下四形四线虫养分去除和生物量生产的影响。结果表明，化学需氧量（CODcr）、NH ₄ ⁺初级出水 (PE) 中的 -N 和总磷 (TP) 分别为 93.9 %、92.3 % 和 84.8 %。与15 h:9 h（15 h光照和9 h黑暗循环）相比，24 h:0 h时叶绿素a的最大含量达到2797.78 mg/m ^{3 。}_{当CO 2}浓度为200 mL/min时，微藻生物量最大（OD ₆₈₀  = 0.56） ，但CO ₂富集抑制了脱氮。在最佳曝气强度（60 L/h）下，第3天叶绿素a含量最高。NH ₄ ⁺ -N和TP的去除率分别为92.1%和89.7%。