This study aimed to analyze the growth performance, antioxidant activity, serum lipid profile, meat quality, and lipid metabolism of broiler chickens fed mixtures containing Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG). A total of 400 one-day-old broiler chickens were randomly divided into 4 treatment groups of 10 replicates with 10 birds each replicate. The dietary treatments consisted of the control group (fed basal diet), and diets supplemented with Enteromorpha polysaccharide (EP; 400 mg/kg), yeast glycoprotein (YG;400 mg/kg), and EP+YG (200 mg/kg EP + 200 mg/kg YG). Compared with the control group, EP+YG supplementation enhanced growth performance and significantly reduced (P < 0.05) serum total triglyceride (TG), cholesterol (CHOL), and low-density lipoprotein LDL levels, and increased high-density lipoprotein (HDL) levels. Besides, birds fed EP+YG supplemented diet exhibited higher (P < 0.05) serum catalase (CAT), total antioxidant capacity, superoxide dismutase (SOD), and lower malonaldehyde (MDA) activities, and upregulated expressions of related genes, such as nuclear factor-erythroid factor 2-related factor 2 (NRF2), SOD1, and glutathione peroxidase 4 (GPX4) in the liver and intestinal tissues than the control group. Interestingly, higher (P < 0.05) serum SOD and lower MDA contents were observed in the EP+YG group than in either EP or YG group, suggesting a synergetic effect. Breast meat from EP+YG supplemented group had significantly higher redness value (a*), and lower pH24, total saturated fatty acid profiles, C14:0, C16:0, C18:0 fatty acid, atherogenic index, and thrombogenicity index than meat from the control group (P < 0.05). Furthermore, the mRNA expressions of fatty acid synthesis genes were downregulated (P < 0.05), whereas lipid β-oxidation-related genes were upregulated (P < 0.05) in the liver of the EP+YG supplemented group than in the control group. Overall, our data suggest that dietary EP+YG inclusion may have a synergistic effect, and therefore improve growth performance, regulate serum biochemical indexes, enhance antioxidant activity, and modulate lipid metabolism in broilers, indicating that it is a potential feed additive for chickens.

本研究旨在分析饲喂含有浒苔多糖 ( EP ) 和酵母糖蛋白 ( YG )混合物的肉鸡的生长性能、抗氧化活性、血清脂质谱、肉质和脂质代谢。将 400 只 1 日龄肉鸡随机分为 4 个处理组，每组 10 个重复，每个重复 10 只鸡。日粮处理包括对照组（饲喂基础日粮）和添加浒苔多糖（EP；400 mg/kg）、酵母糖蛋白（YG；400 mg/kg）和EP+YG（200 mg/kg EP ）的日粮+ 200 毫克/千克 YG)。与对照组相比，补充 EP+YG 增强了生长性能并显着降低了 ( P< 0.05) 血清总甘油三酯 ( TG )、胆固醇 ( CHOL ) 和低密度脂蛋白 LDL 水平，以及高密度脂蛋白 ( HDL ) 水平升高。此外，饲喂EP+YG日粮的鸡群血清过氧化氢酶（ CAT）、总抗氧化能力、超氧化物歧化酶（SOD）和丙二醛（MDA）活性降低，相关基因表达上调（ P < 0.05） 。因子-红细胞因子2相关因子2（NRF2）、SOD1和谷胱甘肽过氧化物酶4 （GPX4）在肝脏和肠道组织中均高于对照组。有趣的是，更高（P < 0.05) EP+YG 组血清 SOD 和 MDA 含量低于 EP 组或 YG 组，提示有协同作用。EP+YG 补充组的胸肉红度值 (a*) 显着高于肉类，pH24、总饱和脂肪酸谱、C14:0、C16:0、C18:0 脂肪酸、致动脉粥样硬化指数和血栓形成指数显着低于肉类来自对照组（P < 0.05）。此外，脂肪酸合成基因mRNA表达下调（P < 0.05），而脂质β-氧化相关基因表达上调（P< 0.05) 在 EP+YG 补充组的肝脏中比在对照组中。总体而言，我们的数据表明，日粮中添加 EP+YG 可能具有协同作用，从而提高肉鸡的生长性能、调节血清生化指标、增强抗氧化活性和调节脂质代谢，表明它是一种潜在的鸡饲料添加剂。