High-solids enzymatic hydrolysis is a promising technology for biochemical conversion of lignocellulosic biomass. However, it may suffer from low efficiency in stirred-tank reactors due to poor mixing degree and enzyme deactivation by excessive shear rate. Inspired by the efficient mixing of viscous chyme and enzyme during intestinal motility, a biomimetic model with an enzyme secretion boundary was first proposed to explore the mixing enhancement mechanism. The dimensionless number Se_m was established to characterize the secondary flow intensity. The results showed that increasing the segmentation amplitude to 66.7% improved Se_m to 9.5 and the mixing level to 38.5%. Extension of the segmentation period resulted in the reduction of Se_m, thus decreasing the mixing enhancement. The time-averaged mixing level was increased by 76.0% compared with the static tube at a substrate viscosity of 5.0 Pa·s. Furthermore, the shear strain rate was lower than 220.4 s^-1, which was conducive to maintaining enzyme activity.

高固含量酶解是一种很有前景的木质纤维素生化转化技术。然而，由于混合度差和剪切速率过大导致酶失活，在搅拌釜反应器中可能会出现效率低下的问题。受粘性食糜和酶在肠道运动过程中有效混合的启发，首先提出了一种具有酶分泌边界的仿生模型来探索混合增​​强机制。建立无量纲数Se _m来表征二次流强度。结果表明，将分割幅度增加到 66.7% ，将Se _m提高到 9.5，混合水平提高到 38.5%。分割周期的延长导致Se的减少_m，从而降低混合增强。在底物粘度为 5.0 Pa·s 时，与静态管相比，时间平均混合水平提高了 76.0%。此外，剪切应变速率低于220.4 s ^-1，有利于维持酶活性。