There is a great demand for sorting applications related to bio/medical cells, such as sorting tumor-infiltrating immune cells (TIICs) from solid tumors. However, the complex operating process, bulky medical instruments, and skilled people become obstacles. We reported an integrated finger-powered cell-sorting microsystem chip that demonstrated TIICs separation from a suspension of a solid tumor. The chip contains the functions of self-powered supply by a finger pump, staining cells by micromixers, cell sorting by deterministic lateral displacement (DLD), and cell-counting by combining an external microscope. The finger-powered microfluidic actuator integrated with the additional valve module handles different fluids and effectively prevents backflow as desired. The integrated micromixer effectively mixes the stain and cell sample in the design ratio. Then, the stained cell solution and the buffer solution are driven into the DLD area by the same pump module. From the four-stage continuous DLD sorting area, the non-target cells are dispersedly shifted. We demonstrated the finger-powered cell-sorting microsystem chip by using the suspension of solid tumor from CT26-bearing BALB/c mouse as a sample. The high purity and recovery rate of 97.23 % and 88.74 % were characterized by finishing the whole function operation in less than a minute.

对与生物/医学细胞相关的分选应用有很大的需求，例如从实体瘤中分选肿瘤浸润性免疫细胞 (TIIC)。然而，复杂的操作流程、笨重的医疗器械和熟练的技术人员成为障碍。我们报告了一种集成的手指供电的细胞分选微系统芯片，该芯片展示了 TIIC 从实体瘤悬浮液中的分离。该芯片包含手指泵自供电、微混合器染色细胞、确定性横向位移（DLD）细胞分选、结合外置显微镜进行细胞计数等功能。与附加阀模块集成的手指驱动微流体致动器可处理不同的流体并根据需要有效防止回流。集成的微混合器以设计比例有效地混合染色剂和细胞样品。然后，染色的细胞溶液和缓冲溶液由同一个泵模块驱动进入 DLD 区域。从四级连续DLD分选区，非靶细胞分散移位。我们以携带 CT26 的 BALB/c 小鼠的实体瘤悬浮液为样本，展示了手指供电的细胞分选微系统芯片。97.23%和88.74%的高纯度和回收率的特点是在不到一分钟的时间内完成了整个功能操作。我们以携带 CT26 的 BALB/c 小鼠的实体瘤悬浮液为样本，展示了手指供电的细胞分选微系统芯片。97.23%和88.74%的高纯度和回收率的特点是在不到一分钟的时间内完成了整个功能操作。我们以携带 CT26 的 BALB/c 小鼠的实体瘤悬浮液为样本，展示了手指供电的细胞分选微系统芯片。97.23%和88.74%的高纯度和回收率的特点是在不到一分钟的时间内完成了整个功能操作。