An ideal tissue-engineered dermal substitute should possess angiogenesis potential to promote wound healing, antibacterial activity to relieve the bacterial burden on skin, as well as sufficient porosity for air and moisture exchange. In light of this, a glass–ceramic (GC) has been incorporated into chitosan and gelatin electrospun nanofibers (240–360 nm), which MEFs were loaded on it for healing acceleration. The GC was doped with silver to improve the antibacterial activity. The bioactive nanofibrous scaffolds demonstrated antibacterial and superior antibiofilm activities against Gram-negative and Gram-positive bacteria. The nanofibrous scaffolds were biocompatible, hemocompatible, and promoted cell attachment and proliferation. Nanofibrous skin substitutes with or without Ag-doped GC nanoparticles did not induce an inflammatory response and attenuated LPS-induced interleukin-6 release by dendritic cells. The rate of biodegradation of the nanocomposite was similar to the rate of skin regeneration under in vivo conditions. Histopathological evaluation of full-thickness excisional wounds in BALB/c mice treated with mouse embryonic fibroblasts-loaded nanofibrous scaffolds showed enhanced angiogenesis, and collagen synthesis as well as regeneration of the sebaceous glands and hair follicles in vivo.

中文翻译：

---

含有掺银生物活性玻璃陶瓷纳米颗粒的细胞负载水凝胶作为皮肤替代品：抗菌特性、免疫反应和无疤痕皮肤伤口再生


理想的组织工程真皮替代品应具有促进伤口愈合的血管生成潜力、减轻皮肤细菌负担的抗菌活性，以及​​用于空气和水分交换的足够的孔隙率。鉴于此，玻璃陶瓷（GC）已被纳入壳聚糖和明胶电纺纳米纤维（240-360 nm）中，并在其上加载 MEF 以加速愈合。 GC 中掺杂银以提高抗菌活性。生物活性纳米纤维支架对革兰氏阴性和革兰氏阳性细菌表现出抗菌和优异的抗生物膜活性。纳米纤维支架具有生物相容性、血液相容性，并促进细胞附着和增殖。无论是否含有掺银 GC 纳米颗粒的纳米纤维皮肤替代品均不会诱发炎症反应，并且不会减弱 LPS 诱导的树突状细胞释放白细胞介素 6。纳米复合材料的生物降解速率与体内条件下皮肤再生的速率相似。用负载小鼠胚胎成纤维细胞的纳米纤维支架治疗的 BALB/c 小鼠的全层切除伤口的组织病理学评估显示，体内血管生成、胶原蛋白合成以及皮脂腺和毛囊再生增强。