Diverse host factors drive microbial variation in plant-associated environments, whereas their genetic mechanisms remain largely unexplored. To address this, we coupled the analyses of plant genetics and microbiomes in this study. Using 100 tea plant (Camellia sinensis) cultivars, the microbiomes of rhizosphere, root endosphere and phyllosphere showed clear compartment-specific assembly, whereas the subpopulation differentiation of tea cultivars exhibited small effects on microbial variation in each compartment. Through microbiome genome-wide association studies, we examined the interactions between tea genetic loci and microbial variation. Notably, genes related to the cell wall and carbon catabolism were heavily linked to root endosphere microbial composition, whereas genes related to the metabolism of metal ions and small organic molecules were overrepresented in association with rhizosphere microbial composition. Moreover, a set of tea genetic variants, including the cytoskeleton-related formin homology interacting protein 1 gene, were strongly associated with the β-diversity of phyllosphere microbiomes, implying their interactions with the overall structure of microbial communities. Our results create a catalogue of tea genetic determinants interacting with microbiomes and reveal the compartment-specific microbiome assembly driven by host genetics.

中文翻译：

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宿主遗传决定因素驱动茶树微生物组的区室特异性组装


不同的宿主因素驱动植物相关环境中的微生物变异，而它们的遗传机制在很大程度上仍未被探索。为了解决这个问题，我们在本研究中结合了植物遗传学和微生物组的分析。利用100个茶树品种，根际、根内圈和叶际的微生物组表现出明显的区室特异性组装，而茶树品种的亚群分化对每个区室的微生物变异影响较小。通过微生物组全基因组关联研究，我们研究了茶遗传位点与微生物变异之间的相互作用。值得注意的是，与细胞壁和碳分解代谢相关的基因与根内圈微生物组成密切相关，而与金属离子和小有机分子代谢相关的基因则与根际微生物组成相关。此外，一组茶遗传变异，包括细胞骨架相关的福尔明同源相互作用蛋白1基因，与叶际微生物组的β多样性密切相关，这意味着它们与微生物群落的整体结构存在相互作用。我们的结果创建了与微生物组相互作用的茶遗传决定因素的目录，并揭示了由宿主遗传学驱动的隔室特异性微生物组组装。