Lactoferrin improves the immune response and resistance of silver carp, a hematological, light (histochemical and immunohistochemical), fluorescent, and scanning electron microscopic study,Microscopy Research and Technique

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Lactoferrin improves the immune response and resistance of silver carp, a hematological, light (histochemical and immunohistochemical), fluorescent, and scanning electron microscopic study
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-07-25 , DOI: 10.1002/jemt.24208
Soha A Soliman ₁ , Walaa F A Emeish ₂ , Hanan Hassan Abdel-Hafeez ₃

Affiliation

Lactoferrin is a multifunctional protein that has roles in iron metabolism, cell generation and differentiation, and antibacterial, antiviral, and antiparasitic activity. The study aim was to evaluate the effect of dietary lactoferrin powder on the innate immune response and disease resistance of silver carp (Hypophthalmichthys molitrix) infected with Vibrio vulnificus. The study also investigates immune cells in different organs including gills, intestine, spleen, liver, and pancreas using light, scan electron microcopy. Two diet regimes, a basic diet and a 600-mg/kg bovine lactoferrin-supplemented diet were used. The fish were fed for 30 successive days. We included 270 apparently healthy silver carp (H. Molitrix) with an average body weight of 10 ± 2 g/fish. Addition of lactoferrin to the diet led to an increase in the activity of non-specific immune parameters; lymphocytes, and in the monocytes percentage, and in total protein, serum globulin, and albumin, with a corresponding increase in phagocytic activities and indices. The histological analysis revealed increase immune cells such as lymphocytes in intestine, rodlet cells in liver, gills, and pancreas, and the macrophages in liver, pancreas as well as spleen. The lactoferrin-supplemented diet significantly increased survival and disease resistance following V. vulnificus challenge, which seemed to correspond to an enhancement of non-specific immune functions in H. molitrix.

中文翻译：

乳铁蛋白提高鲢鱼的免疫反应和抵抗力，血液学、光（组织化学和免疫组织化学）、荧光和扫描电镜研究

乳铁蛋白是一种多功能蛋白质，在铁代谢、细胞生成和分化以及抗菌、抗病毒和抗寄生虫活性中发挥作用。本研究旨在评价日粮乳铁蛋白粉对感染创伤弧菌的鲢鱼（Hypophthalmichthys molitrix ）的先天免疫反应和抗病能力的影响。该研究还使用光扫描电子显微镜研究了不同器官中的免疫细胞，包括鳃、肠、脾、肝和胰腺。使用了两种饮食方案，基本饮食和 600-mg/kg 牛乳铁蛋白补充饮食。连续喂鱼30天。我们纳入了 270 条明显健康的鲢鱼（H. Molitrix) 平均体重为 10 ± 2 克/鱼。在饮食中添加乳铁蛋白导致非特异性免疫参数的活性增加；淋巴细胞，单核细胞百分比，总蛋白，血清球蛋白和白蛋白，吞噬活性和指数相应增加。组织学分析显示肠内淋巴细胞、肝、鳃和胰脏中的小细胞，以及肝、胰和脾脏中的巨噬细胞等免疫细胞增加。补充乳铁蛋白的饮食显着增加了创伤弧菌攻击后的存活率和抗病性，这似乎对应于H. molitrix中非特异性免疫功能的增强。

更新日期：2022-07-25

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