Curcumin Accelerates the Lateral Motion of DPPC Membranes,Langmuir

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Curcumin Accelerates the Lateral Motion of DPPC Membranes
Langmuir ( IF 3.7 ) Pub Date : 2022-07-25 , DOI: 10.1021/acs.langmuir.2c01250
Veerendra Kumar Sharma _{1,

2} , Jyoti Gupta _{1,

2} , Harish Srinivasan _{1,

2} , Himal Bhatt _{2,

3} , Victoria García Sakai ₄ , Subhankur Mitra _{1,

2}

Affiliation

Curcumin, the main ingredient in turmeric, has attracted attention due to its potential anti-inflammatory, anticancer, wound-healing, and antioxidant properties. Though curcumin efficacy is related to its interaction with biomembranes, there are few reports on the effects of curcumin on the lateral motion of lipids, a fundamental process in the cell membrane. Employing the quasielastic neutron scattering technique, we explore the effects of curcumin on the lateral diffusion of the dipalmotylphosphatidylcholine (DPPC) membrane. Our investigation is also supported by Fourier transform infrared spectroscopy, dynamic light scattering, and calorimetry to understand the interaction between curcumin and the DPPC membrane. It is found that curcumin significantly modulates the packing arrangement and conformations of DPPC lipid, leading to enhanced membrane dynamics. In particular, we find that the presence of curcumin substantially accelerates the DPPC lateral motion in both ordered and fluid phases. The effects are more pronounced in the ordered phase where the lateral diffusion coefficient increases by 23% in comparison to 9% in the fluid phase. Our measurements provide critical insights into molecular mechanisms underlying increased lateral diffusion. In contrast, the localized internal motions of DPPC are barely altered, except for a marginal enhancement observed in the ordered phase. In essence, these findings indicate that curcumin is favorably located at the membrane interface rather than in a transbilayer configuration. Further, the unambiguous evidence that curcumin modulates the membrane dynamics at a molecular level supports a possible action mechanism in which curcumin can act as an allosteric regulator of membrane functionality.

中文翻译：

姜黄素加速 DPPC 膜的横向运动

姜黄素是姜黄中的主要成分，因其潜在的抗炎、抗癌、伤口愈合和抗氧化特性而备受关注。尽管姜黄素的功效与其与生物膜的相互作用有关，但很少有关于姜黄素对脂质横向运动（细胞膜中的基本过程）影响的报道。利用准弹性中子散射技术，我们探讨了姜黄素对二棕榈酰磷脂酰胆碱 (DPPC) 膜横向扩散的影响。我们的研究还得到傅里叶变换红外光谱、动态光散射和量热法的支持，以了解姜黄素和 DPPC 膜之间的相互作用。发现姜黄素显着调节 DPPC 脂质的包装排列和构象，导致增强的膜动力学。特别是，我们发现姜黄素的存在大大加速了有序和流体相中的 DPPC 横向运动。与流体相中的 9% 相比，横向扩散系数增加 23% 的有序相中的效果更为明显。我们的测量为增加横向扩散的分子机制提供了重要的见解。相比之下，DPPC 的局部内部运动几乎没有改变，除了在有序阶段观察到的边缘增强。本质上，这些发现表明姜黄素有利地位于膜界面而不是跨双层结构。更远，

更新日期：2022-07-25

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