An integrated magneto-opto-fluidic biosensor for rapid on-chip assay of respiratory viruses of livestock,Lab on a Chip

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An integrated magneto-opto-fluidic biosensor for rapid on-chip assay of respiratory viruses of livestock
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-07-25 , DOI: 10.1039/d2lc00406b
Qinming Zhang ₁ , Gaurav Rawal ₂ , Jingjing Qian ₁ , Hussam Ibrahim ₁ , Jianqiang Zhang ₂ , Liang Dong _{1,

3} , Meng Lu _{1,

3,

4}

Affiliation

Respiratory disease is one of the most important causes of economic loss in swine production. In the USA, porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) are currently the top two primary viruses causing swine respiratory diseases. The commonly used PCR-based virus detection methods require virus extraction, nucleic acid purification, and detection, which are relatively time-consuming and expensive. This work reports an integrated magneto-opto-fluidic (iMOF) platform, in which antibody functionalized magnetic nanoparticles (MNPs) can enable efficient enrichment of multiple swine respiratory viruses and a photonic crystal (PC) biosensor can transduce the amount of captured MNP–virus nanoparticles to the change of their reflection signatures. Owing to the high refractive index of Fe₂O₃ MNPs, the use of MNPs can significantly enhance the PC sensor output. The proof-of-concept validation involves using antibody-functionalized MNPs to recognize IAV and PRRSV and transferring the formed MNP–virus conjugates onto the surface of the PC biosensors to quantify these viruses. The iMOF platform offers a high sensitivity of 3.5 TCID₅₀ mL⁻¹ and 5.9 TCID₅₀ mL⁻¹ for detecting IAV and PRRSV, respectively, and a rapid turnaround within one hour, including the MNP–virus conjugation, enrichment, and detection. The on-chip virus platform has a great potential for in-field surveillance of viral infections.

中文翻译：

一种集成磁光流体生物传感器，用于快速芯片上检测牲畜呼吸道病毒

呼吸系统疾病是造成养猪生产经济损失的最重要原因之一。在美国，猪繁殖与呼吸综合征病毒（PRRSV）和甲型流感病毒（IAV）是目前引起猪呼吸道疾病的前两种主要病毒。常用的基于PCR的病毒检测方法需要病毒提取、核酸纯化和检测，相对耗时且昂贵。这项工作报告了一个集成磁光流体 (iMOF) 平台，其中抗体功能化磁性纳米粒子 (MNP) 可以有效富集多种猪呼吸道病毒，光子晶体 (PC) 生物传感器可以转换捕获的 MNP 病毒的量纳米粒子的反射特征的变化。_{由于 Fe 2}的高折射率O ₃ MNPs，使用MNPs可以显着增强PC传感器输出。概念验证验证包括使用抗体功能化的 MNP 来识别 IAV 和 PRRSV，并将形成的 MNP-病毒偶联物转移到 PC 生物传感器的表面以量化这些病毒。iMOF 平台提供 3.5 TCID ₅₀ mL ^-1和 5.9 TCID ₅₀ mL ^-1的高灵敏度，分别用于检测 IAV 和 PRRSV，并在一小时内实现快速周转，包括 MNP-病毒结合、富集和检测。片上病毒平台在病毒感染的现场监测方面具有巨大潜力。

更新日期：2022-07-25

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