The Ilha River is one of the main tributaries of the Sinos River, consequently contributing to its ecological impact. The present study aimed to associate the use of different biomarkers in vitro and in vivo to assess the water quality of three points along the Ilha River during 2019. For this purpose, water samples were collected at the source and mouth of the river, and in an intermediate section. In the laboratory, samples were used for in vitro toxicity tests with the V79 cell line, in which we evaluated the protein content and mitochondrial activity through the MTT assay. The results indicated a possible disruption in cellular mitochondrial activity. Acute toxicity tests were also carried out in Danio rerio and Daphnia magna, and a chronic toxicity test was performed in C. elegans. No significant differences were observed in the antioxidant defense enzymes (SOD, CAT and GPx); however, the point of greatest agricultural impact inhibited the activity of muscle AChE in zebrafish. All samples delayed the development of C. elegans, characterizing toxicity. Phosphorus was above the limit established by Brazilian legislation at the three sampling points in two of the three collections, indicating the probable presence of agricultural components in the water. The results alert to the need for the association of different bioindicators and, whenever possible, the use of chronic toxicity tests for the best environmental diagnosis.

伊利亚河是西诺斯河的主要支流之一，因此对其生态影响做出了贡献。本研究旨在将体外和体内不同生物标志物的使用联系起来，以评估 2019 年伊拉河沿岸三个点的水质。为此，在河源和河口采集水样，并在一个中间部分。在实验室中，样本用于V79 细胞系的体外毒性测试，我们通过 MTT 测定评估了蛋白质含量和线粒体活性。结果表明细胞线粒体活性可能受到破坏。还进行了斑马鱼的急性毒性试验和Daphnia magna ，并在秀丽隐杆线虫中进行了慢性毒性试验。在抗氧化防御酶（SOD、CAT 和 GPx）中未观察到显着差异；然而，最大的农业影响点抑制了斑马鱼肌肉 AChE 的活性。所有样品都延迟了秀丽隐杆线虫的发展，表征了毒性。在三个采集点中的两个采集点的三个采样点，磷含量高于巴西立法规定的限值，表明水中可能存在农业成分。结果提醒需要将不同的生物指示物联系起来，并尽可能使用慢性毒性测试来进行最佳环境诊断。