Abstract

Silver nanoparticles (AgNPs) have gained interest as an alternative pharmaceutical agent because of antimicrobial resistance and drug toxicity. Considering the increasing request, eco-friendly, sustainable, and cost-effective synthesis of versatile AgNPs has become necessary. In this study, green-made AgNPs were successfully synthesized using Micromonospora sp. SH121 (Mm-AgNPs). Synthesis was verified by surface plasmon resonance (SPR) peak at 402 nm wavelength in the UV-Visible (UV-Vis) absorption spectrum. Scanning electron microscopy (SEM) analysis depicted that Mm-AgNPs were in the size range of 10–30 nm and spherical. Fourier transform infrared spectroscopy (FTIR) confirmed the existence of bioactive molecules on the surface of nanoparticles. The X-ray diffraction (XRD) analysis revealed the face-centered cubic (fcc) structure of the Mm-AgNPs. Their polydispersity index (PDI) and zeta potential were 0. 284 and −35.3 mV, respectively. Mm-AgNPs (4–32 µg/mL) exhibited strong antimicrobial activity against Bacillus cereus, Enterococcus faecalis, Enterococcus hirae, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas putida, Staphylococcus epidermidis, Streptococcus pneumoniae, and Aspergillus flavus. Mm-AgNPs partially inhibited the biofilm formation in Acinetobacter baumannii, E. coli, K. pneumoniae, and Pseudomonas aeruginosa. Furthermore, results showed that low concentrations of Mm-AgNPs (1 and 10 µg/mL) caused higher cytotoxicity and apoptosis in DU 145 cells than human fibroblast cells. Based on the results, Mm-AgNPs have an excellent potential for treating infectious diseases and prostate cancer.

摘要

由于抗菌素耐药性和药物毒性，银纳米粒子 (AgNPs) 作为替代药物已引起人们的兴趣。考虑到日益增长的需求，环保、可持续且具有成本效益的多功能 AgNPs 合成已成为必要。在这项研究中，使用小单孢菌成功合成了绿色 AgNPssp。SH121 (Mm-AgNPs)。通过紫外-可见 (UV-Vis) 吸收光谱中 402 nm 波长处的表面等离子体共振 (SPR) 峰验证合成。扫描电子显微镜 (SEM) 分析表明，Mm-AgNPs 的尺寸范围为 10–30 nm，呈球形。傅立叶变换红外光谱 (FTIR) 证实了纳米粒子表面存在生物活性分子。X 射线衍射 (XRD) 分析揭示了 Mm-AgNP 的面心立方 (fcc) 结构。它们的多分散指数 (PDI) 和 zeta 电位分别为 0. 284 和 -35.3 mV。Mm-AgNPs (4–32 µg/mL) 对蜡样芽孢杆菌、粪肠球菌、平肠球菌、大肠杆菌、肺炎克雷伯菌、普通变形杆菌、恶臭假单胞菌、表皮葡萄球菌、肺炎链球菌和黄曲霉。Mm-AgNPs 部分抑制鲍曼不动杆菌、大肠杆菌、肺炎克雷伯氏菌和铜绿假单胞菌中生物膜的形成。此外，结果表明，与人成纤维细胞相比，低浓度的 Mm-AgNPs（1 和 10 µg/mL）在 DU 145 细胞中引起更高的细胞毒性和细胞凋亡。基于这些结果，Mm-AgNPs 在治疗传染病和前列腺癌方面具有极好的潜力。