Glioma is one of the most common intracranial malignant tumors worldwide. Since the glioma is invasive and lacks a clear boundary with normal brain tissue, the neurosurgeon can only determine the extent of surgical resection based on empirical experience. Thus, accurately demarcating its boundaries has become a major challenge for surgeons. Owing to the high glycolysis metabolism of glioma cells, the acidification of the extracellular fluid has become an indicator of glioma evolution. Herein, a ratiometric pH-responsive surface-enhanced Raman scattering (SERS) strategy was developed for the rapid identification of glioma boundaries. A sensing chip composed of silver nanoparticles self-assembled film was fabricated, followed by the self-assembly of a pH-responsive SERS reporter, 4-mercaptopyridine (4-MPY). The characteristic SERS peak ratios of 4-MPY change regularly under different pH conditions. The boundary of glioma invasion was determined by measuring the pH of waterdrops infiltrated by interstitial fluids. The technology enables accurate, non-invasive, and rapid determination of local pH, thereby maximizing the removal of tumor tissue while minimizing damage to normal tissue. This technique is more rapid and simple than intraoperative pathological detection and can be possibly used for intraoperative navigation.

胶质瘤是全球最常见的颅内恶性肿瘤之一。由于胶质瘤具有侵袭性，与正常脑组织缺乏明确界限，神经外科医生只能根据经验判断手术切除的范围。因此，准确划定其边界已成为外科医生面临的主要挑战。由于胶质瘤细胞的高糖酵解代谢，细胞外液的酸化已成为胶质瘤进化的指标。在此，开发了一种比率 pH 响应的表面增强拉曼散射 (SERS) 策略，用于快速识别胶质瘤边界。制备了由银纳米粒子自组装膜组成的传感芯片，随后自组装了 pH 响应 SERS 报告基因 4-巯基吡啶 (4-MPY)。4-MPY的特征SERS峰比在不同pH条件下有规律地变化。通过测量间质液浸润的水滴的 pH 值来确定胶质瘤侵袭的边界。该技术能够准确、无创和快速地测定局部 pH 值，从而最大限度地去除肿瘤组织，同时最大限度地减少对正常组织的损伤。该技术比术中病理检测更快速、更简单，可用于术中导航。从而最大限度地去除肿瘤组织，同时最大限度地减少对正常组织的损伤。该技术比术中病理检测更快速、更简单，可用于术中导航。从而最大限度地去除肿瘤组织，同时最大限度地减少对正常组织的损伤。该技术比术中病理检测更快速、更简单，可用于术中导航。