In this work, the design and development of a microfluidic paper-based device (μPAD) for the quantification of total phenolic compounds (TPC) in wines is described. The developed μPAD was based upon the vertical flow concept and the colour reaction used was the known Folin-Ciocalteu reaction using gallic acid as reference phenolic compound. After studying operational parameters, namely type of paper, reagents and sample volume, a dynamic range of 5–50 mg L⁻¹ was obtained with a limit of detection of 1.2 mg L⁻¹. The described device proved to have good precision (relative standard deviation < 5%) and no significant interferences were observed from known compounds present in wines. Furthermore, the stability of the colour product and of the device itself were assessed; the μPAD was stable for 30 days (in the dark at room temperature) and it could be scanned up to 8 h after sample introduction. The developed μPAD pose as a simple method for TPC quantification and was successfully applied to several wine samples including sparkling and table wines with two different approaches: i) using gallic acid as reference compound with standard addition; and ii) using taniraisin with external calibration. The accuracy of the proposed μPAD method was assessed by comparison with the reference spectrophotometric method according to the International Organisation of Vine and Wine (OIV) recommendations.

在这项工作中，描述了用于定量葡萄酒中总酚类化合物 (TPC) 的微流体纸基装置 (μPAD) 的设计和开发。开发的 μPAD 基于垂直流动概念，使用的颜色反应是已知的 Folin-Ciocalteu 反应，使用没食子酸作为参考酚类化合物。在研究了操作参数，即纸张类型、试剂和样品量后，获得了 5-50 mg L ^-1的动态范围，检测限为 1.2 mg L ^-1. 所描述的设备被证明具有良好的精度（相对标准偏差 < 5%），并且没有观察到葡萄酒中存在的已知化合物的显着干扰。此外，评估了颜色产品和设备本身的稳定性；μPAD 可以稳定 30 天（在室温下避光），并且可以在样品引入后长达 8 小时进行扫描。开发的 μPAD 作为一种简单的 TPC 定量方法，并通过两种不同的方法成功应用于包括起泡酒和餐酒在内的几种葡萄酒样品：i）使用没食子酸作为标准添加的参考化合物；ii) 使用带有外部校准的 taniraisin。