A new strategy based on online hyphenation of magnetism-enhanced in-tube solid phase microextraction (ME-SPME) and HPLC was developed for speciation of organotin compounds (OTCs) in water and seafood samples. Task specific porous polymer embedded magnetic nanoparticles (TPM) was fabricated in capillary and utilized as the microextraction column (MEC) of ME-SPME. The implement of magnetic field during extraction duration obviously improved extraction efficiencies of prepared TPM/MEC towards studied TOCs from 48.0-77.0% to 84.2–99.7%. Under the optimized extraction parameters, sensitive and automatic approach for the quantification of TOCs was developed by online hyphenation of ME-SPME and HPLC with fluorescence detection. The limits of detection for water and seafood samples were in the ranges of 0.0030–1.5 μg/L and 0.71–14 μg/kg, respectively. The established method was employed to measure low contents of studied OTCs in actual samples, and the recoveries at different fortified contents varied from 80.2% to 116% with satisfactory reproducibility (RSDs below 10%). In addition, confirmation experiments were adopted to inspect the accuracy of introduced method.

开发了一种基于磁增​​强管内固相微萃取 (ME-SPME) 和 HPLC 的在线连字符的新策略，用于水和海鲜样品中的有机锡化合物 (OTC) 的形态分析。任务特异性多孔聚合物嵌入磁性纳米粒子 (TPM) 在毛细管中制造并用作 ME-SPME 的微萃取柱 (MEC)。在提取期间实施磁场明显提高了制备的 TPM/MEC 对所研究 TOC 的提取效率，从 48.0-77.0% 提高到 84.2-99.7%。在优化的提取参数下，通过ME-SPME和具有荧光检测的HPLC的在线连字符开发了用于TOCs定量的灵敏和自动方法。水和海鲜样品的检测限分别在 0.0030-1.5 μg/L 和 0.71-14 μg/kg 范围内。采用所建立的方法测量实际样品中研究的 OTC 的低含量，不同加标含量的回收率从 80.2% 到 116% 不等，重现性良好（RSD 低于 10%）。此外，通过验证实验来检验所介绍方法的准确性。