Molecular encoding in abiotic sequence-defined polymers (SDPs) has recently emerged as a versatile platform for information and data storage. However, the storage capacity of these sequence-defined polymers remains underwhelming compared to that of the information storing biopolymer DNA. In an effort to increase their information storage capacity, herein we describe the synthesis and simultaneous sequencing of eight sequence-defined 10-mer oligourethanes. Importantly, we demonstrate the use of different isotope labels, such as halogen tags, as a tool to deconvolute the complex sequence information found within a heterogeneous mixture of at least 96 unique molecules, with as little as four micromoles of total material. In doing so, relatively high-capacity data storage was achieved: 256 bits in this example, the most information stored in a single sample of abiotic SDPs without the use of long strands. Within the sequence information, a 256-bit cipher key was stored and retrieved. The key was used to encrypt and decrypt a plain text document containing The Wonderful Wizard of Oz. To validate this platform as a medium of molecular steganography and cryptography, the cipher key was hidden in the ink of a personal letter, mailed to a third party, extracted, sequenced, and deciphered successfully in the first try, thereby revealing the encrypted document.

中文翻译：

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使用同时测序、序列定义的低聚聚氨酯混合物进行分子加密和隐写术


非生物序列定义聚合物（SDP）中的分子编码最近已成为信息和数据存储的多功能平台。然而，与存储信息的生物聚合物 DNA 相比，这些序列定义的聚合物的存储容量仍然不足。为了提高其信息存储容量，本文描述了八种序列定义的 10 聚体寡聚氨基甲酸酯的合成和同步测序。重要的是，我们演示了使用不同的同位素标签（例如卤素标签）作为一种工具，可以对至少 96 个独特分子的异质混合物中发现的复杂序列信息进行解卷积，总材料少至 4 微摩尔。通过这样做，实现了相对高容量的数据存储：在本例中为 256 位，这是在不使用长链的情况下存储在非生物 SDP 的单个样本中的最多信息。在序列信息中，存储和检索了 256 位密钥。该密钥用于加密和解密包含《绿野仙踪》的纯文本文档。为了验证该平台作为分子隐写术和密码学的媒介，密钥被隐藏在私人信件的墨水中，邮寄给第三方，在第一次尝试中成功提取、排序和破译，从而揭示了加密的文档。