A dual-emission fluorescent nanoprobe was successfully constructed through self-assembling CsPbBr₃ perovskite nanocrystals (CsPbBr₃ PNCs) and tetraphenylporphyrin tetrasulfonic acid (TPPS). Acetylcholinesterase (AChE) is observed to directly quench the green fluorescence of CsPbBr₃ PNCs at 520 nm in the absence of an enzyme substrate, but has no significant influence on the red emission of TPPS at 650 nm. The decreased value of the fluorescence intensities ratio at 520 to 650 nm (ΔF₅₂₀/F₆₅₀) is proportional to the logarithmic value of AChE activity ranging from 0.05 to 1.0 U/L. The limit of detection is as low as 0.0042 U/L. The relative standard deviation is 3.6% in eleven consecutive measurements of 0.2 U/L AChE. The method exhibits a good anti-interference capacity since it does not respond to most concomitant species. Satisfactory results are acquired for the determination of AChE activity in human serum samples.

_{通过自组装CsPbBr 3}钙钛矿纳米晶（CsPbBr ₃ PNCs）和四苯基卟啉四磺酸（TPPS）成功构建了双发射荧光纳米探针。观察到乙酰胆碱酯酶 (AChE)在没有酶底物的情况下直接淬灭 CsPbBr _{3 PNCs 在 520 nm 处的绿色荧光，但对 TPPS 在 650 nm 处的红色发射没有显着影响。}在 520 到 650 nm 处荧光强度比的降低值 (ΔF ₅₂₀ /F ₆₅₀) 与范围从 0.05 到 1.0 U/L 的 AChE 活性的对数值成正比。检测限低至 0.0042 U/L。在 0.2 U/L AChE 的 11 次连续测量中，相对标准偏差为 3.6%。该方法表现出良好的抗干扰能力，因为它对大多数伴随物种没有反应。人血清样品中 AChE 活性的测定获得了令人满意的结果。