An imported case of monkeypox was diagnosed in December 2019 in a traveller returning from Nigeria to the UK. Subsequently, environmental sampling was performed at two adjoining single-room residences occupied by the patient and their sibling. Monkeypox virus DNA was identified in multiple locations throughout both properties, and monkeypox virus was isolated from several samples 3 days after the patient was last in these locations. Positive samples were identified following the use of both vacuum and surface sampling techniques; these methodologies allowed for environmental analysis of potentially contaminated porous and non-porous surfaces via real-time quantitative reverse transcriptase PCR analysis in addition to viral isolation to confirm the presence of infection-competent virus. This report confirms the potential for infection-competent monkeypox virus to be recovered in environmental settings associated with known positive cases and the necessity for rapid environmental assessment to reduce potential exposure to close contacts and the general public. The methods adopted in this investigation may be used for future confirmed cases of monkeypox in order to establish levels of contamination, confirm the presence of infection-competent material and to identify locations requiring additional cleaning.

中文翻译：

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在英国输入猴痘病例后，在国内环境中发现具有感染能力的猴痘病毒污染

2019 年 12 月，一名从尼日利亚返回英国的旅行者被诊断出一例输入性猴痘病例。随后，在患者及其兄弟姐妹居住的两个相邻的单间住宅中进行了环境采样。猴痘病毒 DNA 在这两个属性的多个位置被识别，并且在患者最后一次出现在这些位置后 3 天从几个样本中分离出猴痘病毒。在使用真空和表面采样技术后，确定了阳性样本；这些方法允许通过实时定量逆转录酶 PCR 分析对潜在污染的多孔和无孔表面进行环境分析，此外还可以进行病毒分离，以确认是否存在具有感染能力的病毒。该报告证实了在与已知阳性病例相关的环境中恢复具有感染能力的猴痘病毒的可能性，以及进行快速环境评估以减少与密切接触者和公众的潜在接触的必要性。本次调查采用的方法可用于未来确诊的猴痘病例，以确定污染程度，确认是否存在感染能力强的材料，并确定需要额外清洁的位置。